Cloning and characterization of a bovine alpha interferon receptor

Jin Kyu Lim, Jerome A. Langer

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

A bovine interferon α receptor (BoIFN-αR1) cDNA, homologous to the human cDNA [1], was isolated. Transfection of the BoIFN-αR1 cDNA into monkey COS cells results in a large increase in high-affinity binding sites for human IFN-αA and IFN-αB. Covalent crosslinking of radiolabeled HuIFN-αA and -αB demonstrates that the complex of [32P]HuIFN with the BoIFN-αR1 protein (predicted mass, 61375) expressed in COS cells migrates as a 140-150 kDa band.

Original languageEnglish (US)
Pages (from-to)314-319
Number of pages6
JournalBBA - Gene Structure and Expression
Volume1173
Issue number3
DOIs
StatePublished - Jun 25 1993

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

Keywords

  • Interferon receptor
  • Protein crosslinking
  • cDNA sequence
  • α-Interferon

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