Cloning and expression of a cDNA encoding the large subunit of duck replication factor C

Ju Tao Guo; Chen Liu; William S. Mason; John C. Pugh

doi:10.1016/S0167-4781(97)00174-7

Cloning and expression of a cDNA encoding the large subunit of duck replication factor C

Ju Tao Guo, Chen Liu, William S. Mason, John C. Pugh

Research output: Contribution to journal › Article › peer-review

Abstract

A cDNA encoding an avian homologue of the large subunit of replication factor C (RFC-L) has been cloned from a duck liver cDNA expression library prepared in bacteriophage lambda. The full length cDNA encodes a protein with a predicted size of approximately 130 kDa, consistent with the size of the polypeptide detected in duck liver. The duck RFC-L amino acid sequence shares 66.4% and 68.4% identity with mouse and human RFC-L proteins, respectively. We identified a 4 kb RFC-L mRNA expressed in most duck tissues.

Original language	English (US)
Pages (from-to)	293-300
Number of pages	8
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1395
Issue number	3
DOIs	https://doi.org/10.1016/S0167-4781(97)00174-7
State	Published - Feb 11 1998
Externally published	Yes

All Science Journal Classification (ASJC) codes

Structural Biology
Biophysics
Biochemistry
Genetics

Keywords

DNA replication
Replication factor C

Access to Document

10.1016/S0167-4781(97)00174-7

Cite this

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title = "Cloning and expression of a cDNA encoding the large subunit of duck replication factor C",

abstract = "A cDNA encoding an avian homologue of the large subunit of replication factor C (RFC-L) has been cloned from a duck liver cDNA expression library prepared in bacteriophage lambda. The full length cDNA encodes a protein with a predicted size of approximately 130 kDa, consistent with the size of the polypeptide detected in duck liver. The duck RFC-L amino acid sequence shares 66.4% and 68.4% identity with mouse and human RFC-L proteins, respectively. We identified a 4 kb RFC-L mRNA expressed in most duck tissues.",

keywords = "DNA replication, Replication factor C",

author = "Guo, {Ju Tao} and Chen Liu and Mason, {William S.} and Pugh, {John C.}",

note = "Funding Information: We are grateful to Heidi Simmons for technical assistance and to Pat Kraus for help in preparing the manuscript. The work was supported by USPHS grants CA-40737 and CA-06927 from the NIH and by an appropriation from the Commonwealth of Pennsylvania.",

year = "1998",

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doi = "10.1016/S0167-4781(97)00174-7",

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T1 - Cloning and expression of a cDNA encoding the large subunit of duck replication factor C

AU - Guo, Ju Tao

AU - Liu, Chen

AU - Mason, William S.

AU - Pugh, John C.

N1 - Funding Information: We are grateful to Heidi Simmons for technical assistance and to Pat Kraus for help in preparing the manuscript. The work was supported by USPHS grants CA-40737 and CA-06927 from the NIH and by an appropriation from the Commonwealth of Pennsylvania.

PY - 1998/2/11

Y1 - 1998/2/11

N2 - A cDNA encoding an avian homologue of the large subunit of replication factor C (RFC-L) has been cloned from a duck liver cDNA expression library prepared in bacteriophage lambda. The full length cDNA encodes a protein with a predicted size of approximately 130 kDa, consistent with the size of the polypeptide detected in duck liver. The duck RFC-L amino acid sequence shares 66.4% and 68.4% identity with mouse and human RFC-L proteins, respectively. We identified a 4 kb RFC-L mRNA expressed in most duck tissues.

AB - A cDNA encoding an avian homologue of the large subunit of replication factor C (RFC-L) has been cloned from a duck liver cDNA expression library prepared in bacteriophage lambda. The full length cDNA encodes a protein with a predicted size of approximately 130 kDa, consistent with the size of the polypeptide detected in duck liver. The duck RFC-L amino acid sequence shares 66.4% and 68.4% identity with mouse and human RFC-L proteins, respectively. We identified a 4 kb RFC-L mRNA expressed in most duck tissues.

KW - DNA replication

KW - Replication factor C

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Cloning and expression of a cDNA encoding the large subunit of duck replication factor C

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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