Cloning and expression of streptococcal pyrogenic exotoxin A and staphylococcal toxic shock syndrome toxin-1 in Bacillus subtilis

B. N. Kreiswirth; J. P. Handley; P. M. Schlievert; R. P. Novick

doi:10.1007/BF00330426

Cloning and expression of streptococcal pyrogenic exotoxin A and staphylococcal toxic shock syndrome toxin-1 in Bacillus subtilis

B. N. Kreiswirth, J. P. Handley, P. M. Schlievert, R. P. Novick

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12 Scopus citations

Abstract

The genes encoding streptococcal pyrogenic exotoxin type A (SPE A) and staphylococcal toxic shock syndrome toxin-1 (TSST-1) were stably cloned and expressed in Bacillus subtilis. In the non-pathogenic Bacillus background, the recombinant speA clone expressed 32-fold more SPE A than the native streptococcus, and similarly, the recombinant plasmid harboring tst expressed 4-fold more TSST-1 in Bacillus than in the native Staphylococcus aureus. The Bacillus-derived products were secreted into the culture fluid, were resistant to proteolytic degradation and their biological activites mimicked native preparations.

Original language	English (US)
Pages (from-to)	84-87
Number of pages	4
Journal	Mgg Molecular & General Genetics
Volume	208
Issue number	1-2
DOIs	https://doi.org/10.1007/BF00330426
State	Published - Jun 1987
Externally published	Yes

All Science Journal Classification (ASJC) codes

Genetics

Keywords

Bacillus expression
Stability
Streptococcal pyrogenic exotoxin A
Toxic shock syndrome toxin-1

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10.1007/BF00330426

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title = "Cloning and expression of streptococcal pyrogenic exotoxin A and staphylococcal toxic shock syndrome toxin-1 in Bacillus subtilis",

abstract = "The genes encoding streptococcal pyrogenic exotoxin type A (SPE A) and staphylococcal toxic shock syndrome toxin-1 (TSST-1) were stably cloned and expressed in Bacillus subtilis. In the non-pathogenic Bacillus background, the recombinant speA clone expressed 32-fold more SPE A than the native streptococcus, and similarly, the recombinant plasmid harboring tst expressed 4-fold more TSST-1 in Bacillus than in the native Staphylococcus aureus. The Bacillus-derived products were secreted into the culture fluid, were resistant to proteolytic degradation and their biological activites mimicked native preparations.",

keywords = "Bacillus expression, Stability, Streptococcal pyrogenic exotoxin A, Toxic shock syndrome toxin-1",

author = "Kreiswirth, {B. N.} and Handley, {J. P.} and Schlievert, {P. M.} and Novick, {R. P.}",

year = "1987",

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T1 - Cloning and expression of streptococcal pyrogenic exotoxin A and staphylococcal toxic shock syndrome toxin-1 in Bacillus subtilis

AU - Kreiswirth, B. N.

AU - Handley, J. P.

AU - Schlievert, P. M.

AU - Novick, R. P.

PY - 1987/6

Y1 - 1987/6

N2 - The genes encoding streptococcal pyrogenic exotoxin type A (SPE A) and staphylococcal toxic shock syndrome toxin-1 (TSST-1) were stably cloned and expressed in Bacillus subtilis. In the non-pathogenic Bacillus background, the recombinant speA clone expressed 32-fold more SPE A than the native streptococcus, and similarly, the recombinant plasmid harboring tst expressed 4-fold more TSST-1 in Bacillus than in the native Staphylococcus aureus. The Bacillus-derived products were secreted into the culture fluid, were resistant to proteolytic degradation and their biological activites mimicked native preparations.

AB - The genes encoding streptococcal pyrogenic exotoxin type A (SPE A) and staphylococcal toxic shock syndrome toxin-1 (TSST-1) were stably cloned and expressed in Bacillus subtilis. In the non-pathogenic Bacillus background, the recombinant speA clone expressed 32-fold more SPE A than the native streptococcus, and similarly, the recombinant plasmid harboring tst expressed 4-fold more TSST-1 in Bacillus than in the native Staphylococcus aureus. The Bacillus-derived products were secreted into the culture fluid, were resistant to proteolytic degradation and their biological activites mimicked native preparations.

KW - Bacillus expression

KW - Stability

KW - Streptococcal pyrogenic exotoxin A

KW - Toxic shock syndrome toxin-1

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Cloning and expression of streptococcal pyrogenic exotoxin A and staphylococcal toxic shock syndrome toxin-1 in Bacillus subtilis

Abstract

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