Cloning and structural analysis of DNA encoding an A2B(1a) subunit of glycinin

Y. A. Marco, V. H. Thanh, Nilgun Tumer, B. J. Scallon, N. C. Nielsen

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The partial DNA sequence of a glycinin gene in a genomic clone and a homologous cDNA clone were determined. They have nearly identical nucleotide sequences and encode the basic polypeptide and part of the acidic polypeptide for an A2B(1a) glycinin subunit. The protein primary structure deduced from the DNA sequence is in close agreement with the amino acid sequence of the subunit determined chemically and confirms assignment of part of the amino acid sequence in the basic component where we were able to establish an overlap using conventional approaches. The coding part of the basic subunit is interrupted by a 625-base pair A + T-rich intron whose boundaries correlate with the established consensus sequences for the exon-intron junctions. Comparison of the nucleotide sequence of the basic subunit of pea legumin gene with that of the gene for A2B(1a) subunit reveals 70% homology in coding regions, although there is considerably less in the 3'-flanking regions.

Original languageEnglish (US)
Pages (from-to)13436-13441
Number of pages6
JournalJournal of Biological Chemistry
Volume259
Issue number21
StatePublished - Dec 1 1984
Externally publishedYes

Fingerprint

Cloning
Structural analysis
Organism Cloning
Genes
DNA sequences
Amino Acid Sequence
Introns
DNA
Nucleotides
3' Flanking Region
Amino Acids
Peptides
Clone Cells
Exons
Peas
Consensus Sequence
Complementary DNA
Base Pairing
glycinin
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Marco, Y. A., Thanh, V. H., Tumer, N., Scallon, B. J., & Nielsen, N. C. (1984). Cloning and structural analysis of DNA encoding an A2B(1a) subunit of glycinin. Journal of Biological Chemistry, 259(21), 13436-13441.
Marco, Y. A. ; Thanh, V. H. ; Tumer, Nilgun ; Scallon, B. J. ; Nielsen, N. C. / Cloning and structural analysis of DNA encoding an A2B(1a) subunit of glycinin. In: Journal of Biological Chemistry. 1984 ; Vol. 259, No. 21. pp. 13436-13441.
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Marco, YA, Thanh, VH, Tumer, N, Scallon, BJ & Nielsen, NC 1984, 'Cloning and structural analysis of DNA encoding an A2B(1a) subunit of glycinin', Journal of Biological Chemistry, vol. 259, no. 21, pp. 13436-13441.

Cloning and structural analysis of DNA encoding an A2B(1a) subunit of glycinin. / Marco, Y. A.; Thanh, V. H.; Tumer, Nilgun; Scallon, B. J.; Nielsen, N. C.

In: Journal of Biological Chemistry, Vol. 259, No. 21, 01.12.1984, p. 13436-13441.

Research output: Contribution to journalArticle

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AU - Thanh, V. H.

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AU - Nielsen, N. C.

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N2 - The partial DNA sequence of a glycinin gene in a genomic clone and a homologous cDNA clone were determined. They have nearly identical nucleotide sequences and encode the basic polypeptide and part of the acidic polypeptide for an A2B(1a) glycinin subunit. The protein primary structure deduced from the DNA sequence is in close agreement with the amino acid sequence of the subunit determined chemically and confirms assignment of part of the amino acid sequence in the basic component where we were able to establish an overlap using conventional approaches. The coding part of the basic subunit is interrupted by a 625-base pair A + T-rich intron whose boundaries correlate with the established consensus sequences for the exon-intron junctions. Comparison of the nucleotide sequence of the basic subunit of pea legumin gene with that of the gene for A2B(1a) subunit reveals 70% homology in coding regions, although there is considerably less in the 3'-flanking regions.

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Marco YA, Thanh VH, Tumer N, Scallon BJ, Nielsen NC. Cloning and structural analysis of DNA encoding an A2B(1a) subunit of glycinin. Journal of Biological Chemistry. 1984 Dec 1;259(21):13436-13441.