Cloning of a major heparan sulfate proteoglycan from brain and identification as the rat form of glypican

L. Karthikeyan, P. Maurel, U. Rauch, R. K. Margolis, R. U. Margolis

Research output: Contribution to journalArticlepeer-review

51 Scopus citations

Abstract

We have obtained the complete coding sequence of a highly conserved heparan sulfate proteoglycan which we previously characterized biochemically after isolation from rat brain. An open reading frame of 558 amino acids encodes a protein with a molecular mass of 62 kDa containing three peptide sequences present in the isolated proteoglycan. The total sequence obtained is 3.5 kb long, including 1.6 kb of 3′-untranslated sequence and 0.2 kb of 5′-untranslated sequence. The deduced amino acid sequence and the 3′- and 5′-untranslated sequences have 89% and 66-80% identity, respectively, with those of a phosphatidylinositol-anchored human lung fibroblast heparan sulfate proteoglycan (glypican) for which mRNA is detectable in a large number of human cell lines. Our data therefore demonstrate that this major heparan sulfate proteoglycan of brain is the rat form of glypican.

Original languageEnglish (US)
Pages (from-to)395-401
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume188
Issue number1
DOIs
StatePublished - Oct 15 1992
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'Cloning of a major heparan sulfate proteoglycan from brain and identification as the rat form of glypican'. Together they form a unique fingerprint.

Cite this