Cloning of chromosome I DNA from Saccharomyces cerevisiae: mutational analysis of the FUN2 transcribed region

Joan C. Crowley, David B. Kaback

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

To increase the number of mutationally defined genes on chromosome I from Saccharomyces cerevisiae, most of the FUN2 (Function Unknown Now) transcribed region was deleted by gene replacement. Strains containing the deletion were viable, but grew with a 20% longer generation time. The mutation was recessive. Mutant haploids were able to mate, and homozygous mutant diploids were able to sporulate, giving asci containing four viable ascospores. These results indicate FUN2 is dispensible for the life cycle of S. cerevisiae, but required for an optimal growth rate.

Original languageEnglish (US)
Pages (from-to)381-385
Number of pages5
JournalGene
Volume83
Issue number2
DOIs
StatePublished - Nov 30 1989

All Science Journal Classification (ASJC) codes

  • Genetics

Keywords

  • Recombinant DNA
  • dispensable gene
  • gene replacement
  • yeast

Fingerprint

Dive into the research topics of 'Cloning of chromosome I DNA from Saccharomyces cerevisiae: mutational analysis of the FUN2 transcribed region'. Together they form a unique fingerprint.

Cite this