Cloning of Pf3, a filamentous bacteriophage of Pseudomonas aeruginosa, into the pBD214 vector of Bacillus subtilis

D. G. Putterman, T. J. Gryczan, D. Dubnau, L. A. Day

Research output: Contribution to journalArticle

Abstract

The genome of Pf3, a filamentous single-stranded DNA bacteriophage of Pseudomonas aeruginosa (a gram-negative organism) was cloned into pBD214, a plasmid cloning vector of Bacillus subtilis (a gram-positive organism). Cloning in the gram-positive organism was done to avoid anticipated lethal effects. The entire Pf3 genome was inserted in each orientation at a unique BclI site within a thymidylate synthetase gene (from B. subtilis phage β22) on the plasmid. Additional clones were made by inserting EcoRI fragments of Pf3 DNA into a unique EcoRI site within this gene.

Original languageEnglish (US)
Pages (from-to)221-223
Number of pages3
JournalJournal of virology
Volume47
Issue number1
DOIs
StatePublished - 1983

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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