TY - JOUR
T1 - Cloning of the Aspergillus parasiticus apa-2 gene associated with the regulation of aflatoxin biosynthesis
AU - Chang, P. K.
AU - Cary, J. W.
AU - Bhatnagar, D.
AU - Cleveland, T. E.
AU - Bennett, J. W.
AU - Linz, J. E.
AU - Woloshuk, C. P.
AU - Payne, G. A.
PY - 1993
Y1 - 1993
N2 - An Aspergillus parasiticus gene, designated apa-2, was identified as a regulatory gene associated with aflatoxin biosynthesis. The apa-2 gene was cloned on the basis of overproduction of pathway intermediates following transformation of fungal strains with cosmid DNA containing the aflatoxin biosynthetic genes nor-1 and ver-1. Transformation of an O- methylsterigmatocystin-accumulating strain, A. parasiticus SRRC 2043, with a 5.5-kb HindIII-XbaI DNA fragment containing apa-2 resulted in overproduction of all aflatoxin pathway intermediates analyzed. Specific enzyme activities associated with the conversion of norsolorinic acid and sterigmatocystin were increased approximately twofold. The apa-2 gene was found to complement an A. flavus afl-2 mutant strain for aflatoxin production, suggesting that apa-2 is functionally homologous to afl-2. Comparison of the A. parasiticus apa-2 gene DNA sequence with that of the A. flavus afl-2 gene (G. A. Payne, G. J. Nystorm, D. Bhatnagar, T. E. Cleveland, and C. P. Woloshuk, Appl. Environ. Microbiol. 59:156-162, 1993) showed that they shared >95% DNA homology. Physical mapping of cosmid subclones placed apa-2 approximately 8 kb from ver-1.
AB - An Aspergillus parasiticus gene, designated apa-2, was identified as a regulatory gene associated with aflatoxin biosynthesis. The apa-2 gene was cloned on the basis of overproduction of pathway intermediates following transformation of fungal strains with cosmid DNA containing the aflatoxin biosynthetic genes nor-1 and ver-1. Transformation of an O- methylsterigmatocystin-accumulating strain, A. parasiticus SRRC 2043, with a 5.5-kb HindIII-XbaI DNA fragment containing apa-2 resulted in overproduction of all aflatoxin pathway intermediates analyzed. Specific enzyme activities associated with the conversion of norsolorinic acid and sterigmatocystin were increased approximately twofold. The apa-2 gene was found to complement an A. flavus afl-2 mutant strain for aflatoxin production, suggesting that apa-2 is functionally homologous to afl-2. Comparison of the A. parasiticus apa-2 gene DNA sequence with that of the A. flavus afl-2 gene (G. A. Payne, G. J. Nystorm, D. Bhatnagar, T. E. Cleveland, and C. P. Woloshuk, Appl. Environ. Microbiol. 59:156-162, 1993) showed that they shared >95% DNA homology. Physical mapping of cosmid subclones placed apa-2 approximately 8 kb from ver-1.
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U2 - 10.1128/aem.59.10.3273-3279.1993
DO - 10.1128/aem.59.10.3273-3279.1993
M3 - Article
C2 - 8250554
AN - SCOPUS:0027381025
SN - 0099-2240
VL - 59
SP - 3273
EP - 3279
JO - Applied and environmental microbiology
JF - Applied and environmental microbiology
IS - 10
ER -