Cloning of the herpes simplex virus type 1 genome as a novel luciferase-tagged infectious bacterial artificial chromosome

You Li, Shuai Wang, Hua Zhu, Chunfu Zheng

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Herpes simplex virus type 1 (HSV-1) is a ubiquitous human pathogen of skin and mucous membranes. In the present study, the genome of the HSV-1 F strain was cloned as an infectious bacterial artificial chromosome (BAC) clone without any deletions of the viral genes. Additionally, a firefly luciferase cassette was inserted to generate a novel luciferase-expressing HSV-1 BAC. Importantly, the resulting recombinant HSV-1 BAC Luc behaved indistinguishably from the wild-type virus in Vero cells, and the luciferase activity could be easily quantified in vitro. Thus, this novel HSV-1 BAC system would serve as a powerful tool for gene function profiling.

Original languageEnglish (US)
Pages (from-to)2267-2272
Number of pages6
JournalArchives of Virology
Volume156
Issue number12
DOIs
StatePublished - Dec 1 2011

All Science Journal Classification (ASJC) codes

  • Virology

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