ClpP of Bacillus subtilis is required for competence development, motility, degradative enzyme synthesis, growth at high temperature and sporulation

Tarek Msadek, Véronique Dartois, Frank Kunst, Marie Laure Herbaud, François Denizot, Georges Rapoport

Research output: Contribution to journalArticle

177 Citations (Scopus)

Abstract

The nucleotide sequence of the Bacillus subtilis clpP gene was determined. The predicted protein shows very high similarity to members of the ClpP family of proteolytic subunits (68% amino acid sequence identity with that of Escherichia coli). We show that ClpP plays an essential role in stationary phase adaptive responses. Indeed, a ΔclpP mutant was constructed and shown to display a pleiotropic phenotype, including a deficiency in both sporulation initiation and competence for DNA uptake. The ΔclpP mutant has a highly filamentous morphology and appears to be non-motile, as judged by swarm plate assays. Expression of clpP is strongly induced under heat shock conditions, and ClpP is shown to be essential for growth of B. subtilis at high temperature. The role of ClpP in the sporulation and competence regulatory pathways was investigated. ClpP is required for expression of the spollA and spollG operons, encoding the σ(F) and σ(E) sporulation-specific sigma factors. ClpP is also necessary for the expression of the comK gene, encoding a positive transcriptional regulator of competence genes. ComK- dependent transcription of sacB, encoding the exocellular degradative enzyme levansucrase, was found to be abolished in the ΔclpP mutant. MecA has been characterized previously as a negative regulator of comK expression, whose overproduction inhibits both sporulation and competence development. Expression of a mecA'-lacZ translational fusion is shown to be increased in the ΔclpP mutant. We suggest that ClpP is involved in controlling MecA levels in the cell through proteolysis. Increased levels of MecA in the absence of ClpP are at least partly responsible for the observed pleiotropic phenotype of the ΔclpP mutant.

Original languageEnglish (US)
Pages (from-to)899-914
Number of pages16
JournalMolecular microbiology
Volume27
Issue number5
DOIs
StatePublished - Jun 15 1998
Externally publishedYes

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Bacillus subtilis
Mental Competency
Temperature
Enzymes
Growth
levansucrase
Phenotype
Regulator Genes
Operon
Proteolysis
Amino Acid Sequence
Shock
Hot Temperature
Escherichia coli
Gene Expression
DNA
Genes
Proteins

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Msadek, Tarek ; Dartois, Véronique ; Kunst, Frank ; Herbaud, Marie Laure ; Denizot, François ; Rapoport, Georges. / ClpP of Bacillus subtilis is required for competence development, motility, degradative enzyme synthesis, growth at high temperature and sporulation. In: Molecular microbiology. 1998 ; Vol. 27, No. 5. pp. 899-914.
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abstract = "The nucleotide sequence of the Bacillus subtilis clpP gene was determined. The predicted protein shows very high similarity to members of the ClpP family of proteolytic subunits (68{\%} amino acid sequence identity with that of Escherichia coli). We show that ClpP plays an essential role in stationary phase adaptive responses. Indeed, a ΔclpP mutant was constructed and shown to display a pleiotropic phenotype, including a deficiency in both sporulation initiation and competence for DNA uptake. The ΔclpP mutant has a highly filamentous morphology and appears to be non-motile, as judged by swarm plate assays. Expression of clpP is strongly induced under heat shock conditions, and ClpP is shown to be essential for growth of B. subtilis at high temperature. The role of ClpP in the sporulation and competence regulatory pathways was investigated. ClpP is required for expression of the spollA and spollG operons, encoding the σ(F) and σ(E) sporulation-specific sigma factors. ClpP is also necessary for the expression of the comK gene, encoding a positive transcriptional regulator of competence genes. ComK- dependent transcription of sacB, encoding the exocellular degradative enzyme levansucrase, was found to be abolished in the ΔclpP mutant. MecA has been characterized previously as a negative regulator of comK expression, whose overproduction inhibits both sporulation and competence development. Expression of a mecA'-lacZ translational fusion is shown to be increased in the ΔclpP mutant. We suggest that ClpP is involved in controlling MecA levels in the cell through proteolysis. Increased levels of MecA in the absence of ClpP are at least partly responsible for the observed pleiotropic phenotype of the ΔclpP mutant.",
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ClpP of Bacillus subtilis is required for competence development, motility, degradative enzyme synthesis, growth at high temperature and sporulation. / Msadek, Tarek; Dartois, Véronique; Kunst, Frank; Herbaud, Marie Laure; Denizot, François; Rapoport, Georges.

In: Molecular microbiology, Vol. 27, No. 5, 15.06.1998, p. 899-914.

Research output: Contribution to journalArticle

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T1 - ClpP of Bacillus subtilis is required for competence development, motility, degradative enzyme synthesis, growth at high temperature and sporulation

AU - Msadek, Tarek

AU - Dartois, Véronique

AU - Kunst, Frank

AU - Herbaud, Marie Laure

AU - Denizot, François

AU - Rapoport, Georges

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N2 - The nucleotide sequence of the Bacillus subtilis clpP gene was determined. The predicted protein shows very high similarity to members of the ClpP family of proteolytic subunits (68% amino acid sequence identity with that of Escherichia coli). We show that ClpP plays an essential role in stationary phase adaptive responses. Indeed, a ΔclpP mutant was constructed and shown to display a pleiotropic phenotype, including a deficiency in both sporulation initiation and competence for DNA uptake. The ΔclpP mutant has a highly filamentous morphology and appears to be non-motile, as judged by swarm plate assays. Expression of clpP is strongly induced under heat shock conditions, and ClpP is shown to be essential for growth of B. subtilis at high temperature. The role of ClpP in the sporulation and competence regulatory pathways was investigated. ClpP is required for expression of the spollA and spollG operons, encoding the σ(F) and σ(E) sporulation-specific sigma factors. ClpP is also necessary for the expression of the comK gene, encoding a positive transcriptional regulator of competence genes. ComK- dependent transcription of sacB, encoding the exocellular degradative enzyme levansucrase, was found to be abolished in the ΔclpP mutant. MecA has been characterized previously as a negative regulator of comK expression, whose overproduction inhibits both sporulation and competence development. Expression of a mecA'-lacZ translational fusion is shown to be increased in the ΔclpP mutant. We suggest that ClpP is involved in controlling MecA levels in the cell through proteolysis. Increased levels of MecA in the absence of ClpP are at least partly responsible for the observed pleiotropic phenotype of the ΔclpP mutant.

AB - The nucleotide sequence of the Bacillus subtilis clpP gene was determined. The predicted protein shows very high similarity to members of the ClpP family of proteolytic subunits (68% amino acid sequence identity with that of Escherichia coli). We show that ClpP plays an essential role in stationary phase adaptive responses. Indeed, a ΔclpP mutant was constructed and shown to display a pleiotropic phenotype, including a deficiency in both sporulation initiation and competence for DNA uptake. The ΔclpP mutant has a highly filamentous morphology and appears to be non-motile, as judged by swarm plate assays. Expression of clpP is strongly induced under heat shock conditions, and ClpP is shown to be essential for growth of B. subtilis at high temperature. The role of ClpP in the sporulation and competence regulatory pathways was investigated. ClpP is required for expression of the spollA and spollG operons, encoding the σ(F) and σ(E) sporulation-specific sigma factors. ClpP is also necessary for the expression of the comK gene, encoding a positive transcriptional regulator of competence genes. ComK- dependent transcription of sacB, encoding the exocellular degradative enzyme levansucrase, was found to be abolished in the ΔclpP mutant. MecA has been characterized previously as a negative regulator of comK expression, whose overproduction inhibits both sporulation and competence development. Expression of a mecA'-lacZ translational fusion is shown to be increased in the ΔclpP mutant. We suggest that ClpP is involved in controlling MecA levels in the cell through proteolysis. Increased levels of MecA in the absence of ClpP are at least partly responsible for the observed pleiotropic phenotype of the ΔclpP mutant.

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