Clustering and co-localization of immunogold double labelled neural cell adhesion molecule isoforms in chick forebrain

Dmitri A. Rusakov, Heather A. Davies, Michael G. Stewart, Melitta Schachner

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

A quantitative immunoelectron microscopic study was carried out in the intermediate portion of hyperstriatum ventrale of chick forebrain (a region exhibiting learning-related morphological plasticity) in order to examine the fine distribution of the neural cell adhesion molecule (N-CAM). Antibodies against a2,8 polysialic acid (PSA), characteristic for embryonic N-CAM, and those against the protein backbones of all N-CAM isoforms were sequentially labelled (post-embedding) with 5- and 15-nm gold particles, and binding to their epitopes was analyzed using the statistics of point processes. The results showed that: (1) PSA tends to form clusters (up to 200 nm in size), in contrast to the protein backbones of all N-CAM isoforms, and (2) the two tissue bound antibodies are colocalized spatially in clusters of sizes up to 100 nm. The data suggest that there is a spatial sub-cellular domain enriched in both PSA and protein isoforms of N-CAM.

Original languageEnglish (US)
Pages (from-to)50-53
Number of pages4
JournalNeuroscience Letters
Volume183
Issue number1-2
DOIs
StatePublished - Jan 2 1995
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Keywords

  • Chick forebrain
  • Double immunogold labelling
  • Neural cell adhesion molecules
  • Sialic acid
  • Statistics of point processes

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