Coagulases as determinants of protective immune responses against Staphylococcus aureus

Molly McAdow, Andrea C. DeDent, Carla Emolo, Alice G. Cheng, Barry N. Kreiswirth, Dominique M. Missiakas, Olaf Schneewinda

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

During infection, Staphylococcus aureus secretes two coagulases (Coa and von Willebrand factor binding protein [vWbp]), which, following an association with host prothrombin and fibrinogen, form fibrin clots and enable the establishment of staphylococcal disease. Within the genomes of different S. aureus isolates, coagulase gene sequences are variable, and this has been exploited for a classification of types. We show here that antibodies directed against the variable prothrombin binding portion of coagulases confer type-specific immunity through the neutralization of S. aureus clotting activity and protection from staphylococcal disease in mice. By combining variable portions of coagulases from North American isolates into hybrid Coa and vWbp proteins, a subunit vaccine that provided protection against challenge with different coagulase-type S. aureus strains in mice was derived.

Original languageEnglish (US)
Pages (from-to)3389-3398
Number of pages10
JournalInfection and immunity
Volume80
Issue number10
DOIs
StatePublished - Oct 2012
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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