Coamplification of 3-hydroxy-3-methylglutaryl coenzyme A reductase genes in methotrexate-resistant human leukemia cell lines

Srinivasan Srimatkandada; Shyam K. Dube; Mark Carmen; Joseph R. Bertino

doi:10.3727/000000001108747390

Coamplification of 3-hydroxy-3-methylglutaryl coenzyme A reductase genes in methotrexate-resistant human leukemia cell lines

Srinivasan Srimatkandada, Shyam K. Dube, Mark Carmen, Joseph R. Bertino

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Methotrexate (MTX)-resistant K562 human myclocytic leukemia sublines with 20- and 200-fold amplified dihydrofolate reductase (DHFR) genes localized to homogeneously staining regions (HSRs) on the long arms of chromosomes 5, 6, and 19 were used to examine whether other genes mapping close to the DHFR genes were coamplified. The gene for 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, located on chromosome 5q13.3-14, was coamplified 4-14-fold, corresponding to the levels of resistance exhibited by these cells. Similar observations were made with a MTX-resistant subline of the promyelocytic leukemia cell line, HL-60R, with 200 gene copies of DHFR. These observations indicate a light linkage of DHFR and HMG-CoA genes on chromosome 5q.

Original language	English (US)
Pages (from-to)	11-15
Number of pages	5
Journal	Oncology Research
Volume	12
Issue number	1
DOIs	https://doi.org/10.3727/000000001108747390
State	Published - 2000
Externally published	Yes

All Science Journal Classification (ASJC) codes

Medicine(all)

Keywords

Coamplification
Dihydrofolate reductase
Methotrexate
Myclocytic leukemia cell lines

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10.3727/000000001108747390

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title = "Coamplification of 3-hydroxy-3-methylglutaryl coenzyme A reductase genes in methotrexate-resistant human leukemia cell lines",

abstract = "Methotrexate (MTX)-resistant K562 human myclocytic leukemia sublines with 20- and 200-fold amplified dihydrofolate reductase (DHFR) genes localized to homogeneously staining regions (HSRs) on the long arms of chromosomes 5, 6, and 19 were used to examine whether other genes mapping close to the DHFR genes were coamplified. The gene for 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, located on chromosome 5q13.3-14, was coamplified 4-14-fold, corresponding to the levels of resistance exhibited by these cells. Similar observations were made with a MTX-resistant subline of the promyelocytic leukemia cell line, HL-60R, with 200 gene copies of DHFR. These observations indicate a light linkage of DHFR and HMG-CoA genes on chromosome 5q.",

keywords = "Coamplification, Dihydrofolate reductase, Methotrexate, Myclocytic leukemia cell lines",

author = "Srinivasan Srimatkandada and Dube, {Shyam K.} and Mark Carmen and Bertino, {Joseph R.}",

year = "2000",

doi = "10.3727/000000001108747390",

language = "English (US)",

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journal = "Oncology Research",

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T1 - Coamplification of 3-hydroxy-3-methylglutaryl coenzyme A reductase genes in methotrexate-resistant human leukemia cell lines

AU - Srimatkandada, Srinivasan

AU - Dube, Shyam K.

AU - Carmen, Mark

AU - Bertino, Joseph R.

PY - 2000

Y1 - 2000

N2 - Methotrexate (MTX)-resistant K562 human myclocytic leukemia sublines with 20- and 200-fold amplified dihydrofolate reductase (DHFR) genes localized to homogeneously staining regions (HSRs) on the long arms of chromosomes 5, 6, and 19 were used to examine whether other genes mapping close to the DHFR genes were coamplified. The gene for 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, located on chromosome 5q13.3-14, was coamplified 4-14-fold, corresponding to the levels of resistance exhibited by these cells. Similar observations were made with a MTX-resistant subline of the promyelocytic leukemia cell line, HL-60R, with 200 gene copies of DHFR. These observations indicate a light linkage of DHFR and HMG-CoA genes on chromosome 5q.

AB - Methotrexate (MTX)-resistant K562 human myclocytic leukemia sublines with 20- and 200-fold amplified dihydrofolate reductase (DHFR) genes localized to homogeneously staining regions (HSRs) on the long arms of chromosomes 5, 6, and 19 were used to examine whether other genes mapping close to the DHFR genes were coamplified. The gene for 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, located on chromosome 5q13.3-14, was coamplified 4-14-fold, corresponding to the levels of resistance exhibited by these cells. Similar observations were made with a MTX-resistant subline of the promyelocytic leukemia cell line, HL-60R, with 200 gene copies of DHFR. These observations indicate a light linkage of DHFR and HMG-CoA genes on chromosome 5q.

KW - Coamplification

KW - Dihydrofolate reductase

KW - Methotrexate

KW - Myclocytic leukemia cell lines

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Coamplification of 3-hydroxy-3-methylglutaryl coenzyme A reductase genes in methotrexate-resistant human leukemia cell lines

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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