Colony formation from protoplasts derived from Douglas fir cotyledons

Edward G. Kirby; Tsai Ying Cheng

doi:10.1016/0304-4211(79)90053-1

Colony formation from protoplasts derived from Douglas fir cotyledons

Edward G. Kirby, Tsai Ying Cheng

Rutgers Newark, Biological Sciences

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

Proliferation of Douglas fir cells regenerated from protoplasts has been accomplished using cotyledons derived from 2-4 week old seedlings. Yield of protoplasts was significantly increased by subjecting cotyledons, prior to protoplast isolation, to a preconditioning treatment for 8 days in the presence of 15 mu;M N₆-benzylaminopurine (BAP) and 500 nM 2-naphthaleneacetic acid (NAA). Protoplasts cultured on a fabric tissue support saturated with a liquid nutrient medium containing a high concentration (10 mM) of glutamine were capable of undergoing rapid cell division leading to colony formation.

Original language	English (US)
Pages (from-to)	145-154
Number of pages	10
Journal	Plant Science Letters
Volume	14
Issue number	2
DOIs	https://doi.org/10.1016/0304-4211(79)90053-1
State	Published - Feb 1979

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title = "Colony formation from protoplasts derived from Douglas fir cotyledons",

abstract = "Proliferation of Douglas fir cells regenerated from protoplasts has been accomplished using cotyledons derived from 2-4 week old seedlings. Yield of protoplasts was significantly increased by subjecting cotyledons, prior to protoplast isolation, to a preconditioning treatment for 8 days in the presence of 15 mu;M N6-benzylaminopurine (BAP) and 500 nM 2-naphthaleneacetic acid (NAA). Protoplasts cultured on a fabric tissue support saturated with a liquid nutrient medium containing a high concentration (10 mM) of glutamine were capable of undergoing rapid cell division leading to colony formation.",

author = "Kirby, {Edward G.} and Cheng, {Tsai Ying}",

note = "Funding Information: Research was supported by a grant from the Weyerhaeuser Corp., Tacoma, WA. Douglas fir seeds were provided by the Weyerhaeuser Forestry Research Center, Centralia, WA. We thank Dr. G. Doyle Daves, Jr., for his critical reading of ~his manuscript and Ms. Faith Henning for growing Douglas fir seedlings.",

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doi = "10.1016/0304-4211(79)90053-1",

language = "English (US)",

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T1 - Colony formation from protoplasts derived from Douglas fir cotyledons

AU - Kirby, Edward G.

AU - Cheng, Tsai Ying

N1 - Funding Information: Research was supported by a grant from the Weyerhaeuser Corp., Tacoma, WA. Douglas fir seeds were provided by the Weyerhaeuser Forestry Research Center, Centralia, WA. We thank Dr. G. Doyle Daves, Jr., for his critical reading of ~his manuscript and Ms. Faith Henning for growing Douglas fir seedlings.

PY - 1979/2

Y1 - 1979/2

N2 - Proliferation of Douglas fir cells regenerated from protoplasts has been accomplished using cotyledons derived from 2-4 week old seedlings. Yield of protoplasts was significantly increased by subjecting cotyledons, prior to protoplast isolation, to a preconditioning treatment for 8 days in the presence of 15 mu;M N6-benzylaminopurine (BAP) and 500 nM 2-naphthaleneacetic acid (NAA). Protoplasts cultured on a fabric tissue support saturated with a liquid nutrient medium containing a high concentration (10 mM) of glutamine were capable of undergoing rapid cell division leading to colony formation.

AB - Proliferation of Douglas fir cells regenerated from protoplasts has been accomplished using cotyledons derived from 2-4 week old seedlings. Yield of protoplasts was significantly increased by subjecting cotyledons, prior to protoplast isolation, to a preconditioning treatment for 8 days in the presence of 15 mu;M N6-benzylaminopurine (BAP) and 500 nM 2-naphthaleneacetic acid (NAA). Protoplasts cultured on a fabric tissue support saturated with a liquid nutrient medium containing a high concentration (10 mM) of glutamine were capable of undergoing rapid cell division leading to colony formation.

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Colony formation from protoplasts derived from Douglas fir cotyledons

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