Colony formation from protoplasts derived from Douglas fir cotyledons

Edward G. Kirby, Tsai Ying Cheng

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33 Scopus citations


Proliferation of Douglas fir cells regenerated from protoplasts has been accomplished using cotyledons derived from 2-4 week old seedlings. Yield of protoplasts was significantly increased by subjecting cotyledons, prior to protoplast isolation, to a preconditioning treatment for 8 days in the presence of 15 mu;M N6-benzylaminopurine (BAP) and 500 nM 2-naphthaleneacetic acid (NAA). Protoplasts cultured on a fabric tissue support saturated with a liquid nutrient medium containing a high concentration (10 mM) of glutamine were capable of undergoing rapid cell division leading to colony formation.

Original languageEnglish (US)
Pages (from-to)145-154
Number of pages10
JournalPlant Science Letters
Issue number2
StatePublished - Feb 1979


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