ComBase Models Are Valid for Predicting Fate of Listeria monocytogenes on 10 Whole Intact Raw Fruits and Vegetables

MARINA GIRBAL, LAURA K. STRAWN, CLAIRE M. MURPHY, CAMERON A. BARDSLEY, DONALD W. SCHAFFNER

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Listeria monocytogenes was associated with more than 60 produce recalls, including tomato, cherry, broccoli, lemon, and lime, between 2017 and 2020. This study describes the effects of temperature, time, and food substrate as factors influencing L. monocytogenes behavior on whole intact raw fruits and vegetables. Ten intact whole fruit and vegetable commodities were chosen based on data gaps identified in a systematic literature review. Produce investigated belong to major commodity families: Ericaceae (blackberry, raspberry, and blueberry), Rutaceae (lemon and mandarin orange), Roseaceae (sweet cherry), Solanaceae (tomato), Brassaceae (cauliflower and broccoli), and Apiaceae (carrot). A cocktail of five L. monocytogenes strains that included clinical, food, or environmental isolates linked to foodborne outbreaks was used to inoculate intact whole fruits and vegetables. Samples were incubated at 2, 12, 22, 30, and 358C with relative humidities matched to typical real-world conditions. Foods were sampled (n=6) for up to 28 days, depending on temperature. Growth and decline rates were estimated using DMFit, an Excel add-in. Growth rates were compared with ComBase modeling predictions for L. monocytogenes. Almost every experiment showed initial growth, followed by subsequent decline. L. monocytogenes was able to grow on the whole intact surface of all produce tested, except for carrot. The 10 produce commodities supported growth of L. monocytogenes at 22 and 358C. Growth and survival at 2 and 128C varied by produce commodity. The standard deviation of the square root growth and decline rates showed significantly larger variability in both growth and decline rates within replicates as temperature increased. When L. monocytogenes growth occurred, it was conservatively modeled by ComBase Predictor, and growth was generally followed by decreases in concentration. This research will assist in understanding the risks of foodborne disease outbreaks and recalls associated with L. monocytogenes on fresh whole produce.

Original languageEnglish (US)
Pages (from-to)597-610
Number of pages14
JournalJournal of food protection
Volume84
Issue number4
DOIs
StatePublished - Apr 2021

All Science Journal Classification (ASJC) codes

  • Food Science
  • Microbiology

Keywords

  • Berries
  • Brassica
  • Carrot
  • Cherries
  • Citrus
  • Tomato

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