We propose an automatic scanning microscope that is capable of analyzing the properties of the biofilm-associated cells by using optical and impedance spectroscopy. The operating principle of the instrument is based on measuring the electrical impedance of cell culture grown on a conductive substratum that is used as one of the electrodes. At low frequencies, the impedance analysis is capable of characterizing a biofilm at the macroscale, and at high frequencies it is capable of analyzing the peculiarities of a cell layer at the level of single microorganisms. The combination of these two techniques is sufficient to give a quantitative and structural composition of a biofilm at both levels. The developed instrument can be useful in the broad range of biofilmrelated research studies, providing the data for detailed, real-time, computer-controlled, noninvasive analysis of cell-to-cell and cell-to-surface interactions.
All Science Journal Classification (ASJC) codes
- Computer Science Applications
- Medical Laboratory Technology