@article{ccaecf4e541d4551b20d544d32810cf4,
title = "Combining mutations in HIV-1 reverse transcriptase with mutations in the HIV-1 polypurine tract affects RNase H cleavages involved in PPT utilization",
abstract = "The RNase H cleavages that generate and remove the polypurine tract (PPT) primer during retroviral reverse transcription must be specific to generate linear viral DNAs that are suitable substrates for the viral integrase. To determine if specific contacts between reverse transcriptase (RT) and the PPT are a critical factor in determining the cleavage specificity of RNase H, we made HIV-1 viruses containing mutations in RT and the PPT at the locations of critical contacts between the protein and the nucleic acid. The effects on titer and RNase H cleavage suggest that combining mutations in RT with mutations in the PPT affect the structure of the protein of the RT/nucleic acid complex in ways that affect the specificity and the rate of PPT cleavage. In contrast, the mutations in the PPT (alone) and RT (alone) affect the specificity of PPT cleavage but have much less effect on the overall rate of cleavage.",
keywords = "HIV-1 reverse transcriptase, Polypurine tract, RNase H cleavage specificity",
author = "McWilliams, {Mary Jane} and Julias, {John G.} and Sarafianos, {Stefan G.} and Alvord, {W. Gregory} and Eddy Arnold and Hughes, {Stephen H.}",
note = "Funding Information: The authors thank Refika Turnier, Elizabeth Shanklin-Selby and Guity Mohammadi for help with FACS and the SAIC-Frederick Laboratory of Molecular Technology, especially Claudia Stewart, for technical support. We also thank Hilda Marusiodis for assistance in manuscript preparation and Mike Abrams for critically reading the manuscript. This publication has been funded in whole or in part with Federal funds from the National Cancer Institute, National Institutes of Health, under Contract No. NO1-CO-12400. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services nor does mention of trade names, commercial products or organizations imply endorsement by the U.S. Government. This research was supported [in part] by the Intramural Research Program of the NIH, National Cancer Institute, Center for Cancer Research.",
year = "2006",
month = may,
day = "10",
doi = "10.1016/j.virol.2005.12.042",
language = "English (US)",
volume = "348",
pages = "378--388",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "2",
}