ComEA is a DNA receptor for transformation of competent Bacillus subtilis

R. Provvedi, D. Dubnau

Research output: Contribution to journalArticlepeer-review

92 Scopus citations

Abstract

Competent cells of Bacillus subtilis efficiently bind and internalize DNA. ComEA and the seven proteins encoded by the comG operon are required in vivo for the binding step. We show here that ComEA, a bitopic membrane protein, is itself capable of high-affinity DNA binding. A domain necessary for DNA binding is located at the C-terminus of ComEA. Proteins with similar 60-80 amino acid residue domains are widespread among bacteria and higher organisms. ComEA shows a marked preference for double-stranded DNA and can bind to oligomers as small as 22 bp in length. DNA binding by ComEA exhibits no apparent base sequence specificity. Using a membrane vesicle DNA-binding assay system we show that in the absence of cell wall, ComEA is still required for DNA binding, whereas the requirement for the ComG proteins is bypassed. We conclude that the ComG proteins are needed in vivo to provide access of the binding domain of ComEA to exogenous DNA. Possible specific roles for the ComG proteins are discussed.

Original languageEnglish (US)
Pages (from-to)271-280
Number of pages10
JournalMolecular microbiology
Volume31
Issue number1
DOIs
StatePublished - 1999

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Fingerprint

Dive into the research topics of 'ComEA is a DNA receptor for transformation of competent Bacillus subtilis'. Together they form a unique fingerprint.

Cite this