ComEB protein is dispensable for the transformation but must be translated for the optimal synthesis of comEC

Micaela De Santis, Jeanette Hahn, David Dubnau

Research output: Contribution to journalArticlepeer-review

Abstract

We show that the ComEB protein is not required for transformation in Bacillus subtilis, despite its expression from within the comE operon under competence control, nor is it required for the correct polar localization of ComGA. We show further that the synthesis of the putative channel protein ComEC is translationally coupled to the upstream comEB open reading frame, so that the translation of comEB and a suboptimal ribosomal-binding site embedded in its sequence are needed for proper comEC expression. Translational coupling appears to be a common mechanism in three major competence operons for the adjustment of protein amounts independent of transcriptional control, probably ensuring the correct stoichiometries for assembly of the transformation machinery. comEB and comFC, respectively, encode cytidine deaminase and a protein resembling type 1 phosphoribosyl transferases and we speculate that nucleotide scavenging proteins are produced under competence control for efficient reutilization of the products of degradation of the non-transforming strand during DNA uptake.

Original languageEnglish (US)
Pages (from-to)71-79
Number of pages9
JournalMolecular microbiology
Volume116
Issue number1
DOIs
StatePublished - Jul 2021

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Keywords

  • ComEB
  • ComEC
  • nucleotide scavenging
  • posttranscriptional regulation
  • transformation
  • translational coupling

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