Comparative Dynamic Stabilities of Cyclopropyl and Olefinic Model Lipids in Liposomes. A Coordinated Kinetic and Spectroscopic Study

Robert A. Moss, Tsunehisa Fujita, Yukihisa Okumura, Zheng Hua, Richard Mendelsohn, Lawrence Senak

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Cyclopropanated dihydrosterculoyl (3-F, 3-NF) and unsaturated oleoyl (cis-2-F, cis-2-NF) ammonium ion lipids were prepared. Liposomes were created by sonication of 1:10 functional/nonfunctional lipid blends. The small (300–450 A) liquid crystalline (Tc 15 °C) liposomes were surface differentiated by exovesicular glutathione in an endovesicvdar/exovesicular pH gradient of 3.9/8. At 25 °C, the cyclopropyl liposomes of 3 reequilibrated by transbilayer lipid migration much more slowly (ti1/2=16 min) than the oleoyl liposomes of 2 (t1/2 <1 min). The origins of this difference were traced to variations in the lipids' acyl chain conformational states. Fourier transform IR studies revealed that the acyl chains of 3 contained fewer highly disruptive double gauche conformational sites than the chains of 2. This can be associated with better chain packing, increased chain order, and the observed enhanced resistance to lipid “flip-flop” in liposomes of 3.

Original languageEnglish (US)
Pages (from-to)1731-1735
Number of pages5
JournalLangmuir
Volume8
Issue number7
DOIs
StatePublished - Jul 1 1992

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

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