Comparative effect of different test methodologies on Bacillus coagulans spores inactivation kinetics in tomato pulp under isothermal conditions

Morgana Zimmermann, Suzane Miorelli, Donald W. Schaffner, Gláucia M.F. Aragão

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Heat resistant micro-organisms are an ongoing challenge to the food industry. Various factors may influence the heat resistance of micro-organisms including type and strain; the environmental influences during cell and spore formations and during heat exposure; and the equipment and test tools used to perform the experimental process. In an attempt to analyse the influence of different test tools used on the heat inactivation processes, this study aimed to define the isothermal inactivation kinetics of Bacillus coagulans spores in tomato pulp at different temperatures and compare the inactivation of this bacterium when thermal death time (TDT) and capillary tube methods were used. Temperature ranges from 95 °C to 120 °C were studied, and inactivation kinetic parameters were estimated through the application of primary models. TDT inactivation curves consisted of shoulder and linear decline, while capillary method inactivation curves consisted of shoulder, linear decline and long tail. A secondary model was used to describe the influence of the temperature on spore inactivation parameters. The results showed test methods are at least as important in determining thermal processes as the micro-organisms and media used.

Original languageEnglish (US)
Pages (from-to)1722-1728
Number of pages7
JournalInternational Journal of Food Science and Technology
Volume48
Issue number8
DOIs
StatePublished - Aug 2013

All Science Journal Classification (ASJC) codes

  • Food Science
  • Industrial and Manufacturing Engineering

Keywords

  • Bacillus coagulans
  • Capillary tube
  • TDT tube
  • Thermal inactivation
  • Tomato pulp

Fingerprint Dive into the research topics of 'Comparative effect of different test methodologies on Bacillus coagulans spores inactivation kinetics in tomato pulp under isothermal conditions'. Together they form a unique fingerprint.

  • Cite this