Comparative genomics of susceptibility to mammary carcinogenesis among inbred rat strains

Role of reduced prolactin signaling in resistance of the Copenhagen strain

Xuefeng Ren, Xun Zhang, Andrea S. Kim, Andrei M. Mikheev, Mingzhu Fang, Robert C. Sullivan, Roger E. Bumgarner, Helmut Zarbl

Research output: Contribution to journalArticle

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Abstract

To elucidate the molecular basis for differential susceptibilities to mammary carcinogenesis, we compared the transcriptomes of normal mammary glands from pubescent female rats of the resistant Copenhagen (Cop) strain with those of the susceptible Fischer 344 (F344), August × Copenhagen Irish (ACI), Buffalo/N (Buf/N), Wistar-Furth (WF) strains and F1 (Cop × F344) progeny (F1). Gene expression profiles in mammary tissue within each rat strain were remarkably similar, indicating that gene expression was determined by genetic background. We next identified the subset of genes that were differentially expressed in all susceptible strains relative to the resistant Cop strain. Among these, the messenger RNAs encoding prolactin (Prl) and its cell surface receptor were significantly elevated in all susceptible strains. The expression levels of several Prl-regulated genes were also significantly elevated, indicating the presence of increased Prl signaling in mammary glands of all susceptible strains. Pathway analysis of gene expression profiles further identified the Prl-activated Jak/STAT -signaling pathway among the pathways that most distinguished sensitive rat strains from the resistant Cop rat. To test the hypothesis that reduced levels of the Prl signaling in mammary tissue partially contributed to the genetic resistance to mammary carcinogenesis, we used the neuroleptic drug, perphenazine, to transiently elevate serum Prl levels in the Cop strain. Whereas Cop rats are resistant to N -nitroso- N -methylurea (NMU)-induced mammary carcinogenesis, ∼5% of pubescent Cop females treated with perphenazine and NMU exposure developed mammary adenocarcinomas with latencies comparable with those of sensitive strains. Together, these finding indicated that in the rat, the molecular mechanisms underlying genetic susceptibility to mammary carcinogenesis include de-regulation of Prl signaling.

Original languageEnglish (US)
Pages (from-to)177-185
Number of pages9
JournalCarcinogenesis
Volume29
Issue number1
DOIs
StatePublished - Jan 1 2008

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Inbred Strains Rats
Genomics
Prolactin
Carcinogenesis
Breast
Transcriptome
Perphenazine
Human Mammary Glands
Buffaloes
Cell Surface Receptors
Genetic Predisposition to Disease
Antipsychotic Agents
Genes
Adenocarcinoma
Gene Expression
Messenger RNA
Serum

All Science Journal Classification (ASJC) codes

  • Cancer Research

Cite this

Ren, Xuefeng ; Zhang, Xun ; Kim, Andrea S. ; Mikheev, Andrei M. ; Fang, Mingzhu ; Sullivan, Robert C. ; Bumgarner, Roger E. ; Zarbl, Helmut. / Comparative genomics of susceptibility to mammary carcinogenesis among inbred rat strains : Role of reduced prolactin signaling in resistance of the Copenhagen strain. In: Carcinogenesis. 2008 ; Vol. 29, No. 1. pp. 177-185.
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abstract = "To elucidate the molecular basis for differential susceptibilities to mammary carcinogenesis, we compared the transcriptomes of normal mammary glands from pubescent female rats of the resistant Copenhagen (Cop) strain with those of the susceptible Fischer 344 (F344), August × Copenhagen Irish (ACI), Buffalo/N (Buf/N), Wistar-Furth (WF) strains and F1 (Cop × F344) progeny (F1). Gene expression profiles in mammary tissue within each rat strain were remarkably similar, indicating that gene expression was determined by genetic background. We next identified the subset of genes that were differentially expressed in all susceptible strains relative to the resistant Cop strain. Among these, the messenger RNAs encoding prolactin (Prl) and its cell surface receptor were significantly elevated in all susceptible strains. The expression levels of several Prl-regulated genes were also significantly elevated, indicating the presence of increased Prl signaling in mammary glands of all susceptible strains. Pathway analysis of gene expression profiles further identified the Prl-activated Jak/STAT -signaling pathway among the pathways that most distinguished sensitive rat strains from the resistant Cop rat. To test the hypothesis that reduced levels of the Prl signaling in mammary tissue partially contributed to the genetic resistance to mammary carcinogenesis, we used the neuroleptic drug, perphenazine, to transiently elevate serum Prl levels in the Cop strain. Whereas Cop rats are resistant to N -nitroso- N -methylurea (NMU)-induced mammary carcinogenesis, ∼5{\%} of pubescent Cop females treated with perphenazine and NMU exposure developed mammary adenocarcinomas with latencies comparable with those of sensitive strains. Together, these finding indicated that in the rat, the molecular mechanisms underlying genetic susceptibility to mammary carcinogenesis include de-regulation of Prl signaling.",
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Comparative genomics of susceptibility to mammary carcinogenesis among inbred rat strains : Role of reduced prolactin signaling in resistance of the Copenhagen strain. / Ren, Xuefeng; Zhang, Xun; Kim, Andrea S.; Mikheev, Andrei M.; Fang, Mingzhu; Sullivan, Robert C.; Bumgarner, Roger E.; Zarbl, Helmut.

In: Carcinogenesis, Vol. 29, No. 1, 01.01.2008, p. 177-185.

Research output: Contribution to journalArticle

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