Comparison of a simplified whole blood and isolated lymphocyte stimulation technique

S. E. Keller; S. J. Schleifer; J. Sherman; M. S. Camerino; H. Smith; M. Stein

doi:10.3109/08820138109050707

Comparison of a simplified whole blood and isolated lymphocyte stimulation technique

S. E. Keller, S. J. Schleifer, J. Sherman, M. S. Camerino, H. Smith, M. Stein

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Whole blood and isolated lymphocyte stimulation in healthy men were compared utilizing PHA, ConA, and PWM. Dose response curves with each of the 3 mitogens were found to differ with the two techniques of lymphocyte stimulation. When incubation time was lengthened PWM responses increased markedly in both the whole blood and isolated lymphocyte assays. Increased incubation time had no effect on PHA cultures and increased the responses with ConA in the whole blood method. In each study, responses with ³H-thymidine were comparable to responses utilizing ¹²⁵IUdR.

Original language	English (US)
Pages (from-to)	417-431
Number of pages	15
Journal	Unknown Journal
Volume	10
Issue number	4-5
DOIs	https://doi.org/10.3109/08820138109050707
State	Published - Jan 1 1981
Externally published	Yes

All Science Journal Classification (ASJC) codes

Immunology

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abstract = "Whole blood and isolated lymphocyte stimulation in healthy men were compared utilizing PHA, ConA, and PWM. Dose response curves with each of the 3 mitogens were found to differ with the two techniques of lymphocyte stimulation. When incubation time was lengthened PWM responses increased markedly in both the whole blood and isolated lymphocyte assays. Increased incubation time had no effect on PHA cultures and increased the responses with ConA in the whole blood method. In each study, responses with 3H-thymidine were comparable to responses utilizing 125IUdR.",

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AU - Keller, S. E.

AU - Schleifer, S. J.

AU - Sherman, J.

AU - Camerino, M. S.

AU - Smith, H.

AU - Stein, M.

PY - 1981/1/1

Y1 - 1981/1/1

N2 - Whole blood and isolated lymphocyte stimulation in healthy men were compared utilizing PHA, ConA, and PWM. Dose response curves with each of the 3 mitogens were found to differ with the two techniques of lymphocyte stimulation. When incubation time was lengthened PWM responses increased markedly in both the whole blood and isolated lymphocyte assays. Increased incubation time had no effect on PHA cultures and increased the responses with ConA in the whole blood method. In each study, responses with 3H-thymidine were comparable to responses utilizing 125IUdR.

AB - Whole blood and isolated lymphocyte stimulation in healthy men were compared utilizing PHA, ConA, and PWM. Dose response curves with each of the 3 mitogens were found to differ with the two techniques of lymphocyte stimulation. When incubation time was lengthened PWM responses increased markedly in both the whole blood and isolated lymphocyte assays. Increased incubation time had no effect on PHA cultures and increased the responses with ConA in the whole blood method. In each study, responses with 3H-thymidine were comparable to responses utilizing 125IUdR.

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