An atmosphere with reduced oxygen tension is required for the primary isolation of Campylobacter fetus subsp. jejuni. Therefore, we compared use of the conventional atmosphere of 5% oxygen and 8% carbon dioxide with use of a candle jar (17% oxygen and 3% carbon dioxide) for primary isolation of C. fetus subsp. jejuni from 263 positive canine, cattle, and turkey fecal or cecal specimens. At an incubation temperature of 42°C, the atmosphere with 5% oxygen resulted in more Campylobacter colonies per plate (P<0.005) and consistently larger Campylobacter colonies (P<0.005) than did the candle jar, whereas the growth of interfering flora was similar. Overall, 96% of the 263 specimens were positive for C. fetus subsp. jejuni with 5% oxygen, and 90% were positive with the candle jar (P<0.02). More striking differences in isolation rates were seen when both the temperature and the atmosphere were varied: 5% oxygen at 42°C enabled recovery of 93% of the isolates from 70 positive specimens, versus 46% recovery with the candle jar at 37°C. Results with 5% oxygen at 37° C were intermediate. The addition of FBP supplement (0.25% each of ferrous sulfate, sodium metabisulfite, and sodium pyruvate) to Campy-BAP selective medium made no improvement over unsupplemented medium at 42°C (whether in 5% oxygen or in the candle jar), but there was significant improvement over unsupplemented medium when both media were incubated at 37°C in the candle jar. We conclude that 5% oxygen is superior to the candle jar for primary isolation of C. fetus subsp. jejuni from animal specimens and that interaction of the two factors tested (temperature and atmosphere of incubation) greatly decreases isolation rates at 37°C in a candle jar.
All Science Journal Classification (ASJC) codes
- Microbiology (medical)