Comparison of epi-GM3 with GM3 and GM1 as stimulators of neurite outgrowth

Michael S. Cannella; Fred J. Roisen; Tomoya Ogawa; Mamoru Sugimoto; Robert W. Ledeen

doi:10.1016/0165-3806(88)90075-2

Comparison of epi-GM3 with GM3 and GM1 as stimulators of neurite outgrowth

Michael S. Cannella, Fred J. Roisen, Tomoya Ogawa, Mamoru Sugimoto, Robert W. Ledeen

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

A variety of naturally occurring ganglioside structures were previously shown to be effective agents for inducing neurite outgrowth of primary neurons and neuroblastoma lines. We report here the results of similar experiments with a synthetic epimer of GM3 (epi-GM3) possessing a neuraminidase-resistant β-ketosidic linkage. This substance was found to enhance neuritogenesis toward two transformed cell lines (neuro-2A, PC-12) and one primary neuronal tissue (dorsal root ganglia). The results indicate that the stereochemistry of the ketoside linkage is not critical and that metabolism of exogenous ganglioside by the treated cells is not involved directly in the neuritogenic phenomenon.

Original language	English (US)
Pages (from-to)	137-143
Number of pages	7
Journal	Developmental Brain Research
Volume	39
Issue number	1
DOIs	https://doi.org/10.1016/0165-3806(88)90075-2
State	Published - Mar 1 1988
Externally published	Yes

All Science Journal Classification (ASJC) codes

Developmental Neuroscience
Developmental Biology

Keywords

Dorsal root ganglion
Epi-GM3
Ganglioside GM3
Neuritogenesis
Neuro-2A cell
PC-12 cell

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10.1016/0165-3806(88)90075-2

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abstract = "A variety of naturally occurring ganglioside structures were previously shown to be effective agents for inducing neurite outgrowth of primary neurons and neuroblastoma lines. We report here the results of similar experiments with a synthetic epimer of GM3 (epi-GM3) possessing a neuraminidase-resistant β-ketosidic linkage. This substance was found to enhance neuritogenesis toward two transformed cell lines (neuro-2A, PC-12) and one primary neuronal tissue (dorsal root ganglia). The results indicate that the stereochemistry of the ketoside linkage is not critical and that metabolism of exogenous ganglioside by the treated cells is not involved directly in the neuritogenic phenomenon.",

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AU - Roisen, Fred J.

AU - Ogawa, Tomoya

AU - Sugimoto, Mamoru

AU - Ledeen, Robert W.

PY - 1988/3/1

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N2 - A variety of naturally occurring ganglioside structures were previously shown to be effective agents for inducing neurite outgrowth of primary neurons and neuroblastoma lines. We report here the results of similar experiments with a synthetic epimer of GM3 (epi-GM3) possessing a neuraminidase-resistant β-ketosidic linkage. This substance was found to enhance neuritogenesis toward two transformed cell lines (neuro-2A, PC-12) and one primary neuronal tissue (dorsal root ganglia). The results indicate that the stereochemistry of the ketoside linkage is not critical and that metabolism of exogenous ganglioside by the treated cells is not involved directly in the neuritogenic phenomenon.

AB - A variety of naturally occurring ganglioside structures were previously shown to be effective agents for inducing neurite outgrowth of primary neurons and neuroblastoma lines. We report here the results of similar experiments with a synthetic epimer of GM3 (epi-GM3) possessing a neuraminidase-resistant β-ketosidic linkage. This substance was found to enhance neuritogenesis toward two transformed cell lines (neuro-2A, PC-12) and one primary neuronal tissue (dorsal root ganglia). The results indicate that the stereochemistry of the ketoside linkage is not critical and that metabolism of exogenous ganglioside by the treated cells is not involved directly in the neuritogenic phenomenon.

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KW - Ganglioside GM3

KW - Neuritogenesis

KW - Neuro-2A cell

KW - PC-12 cell

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Comparison of epi-GM3 with GM3 and GM1 as stimulators of neurite outgrowth

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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