Comparison of germline mosaics of genes in the Polycomb group of Drosophila melanogaster

M. C. Soto, T. B. Chou, W. Bender

Research output: Contribution to journalArticlepeer-review

99 Scopus citations

Abstract

The genes of the Polycomb group (PcG) repress the genes of the bithorax and Antennapedia complexes, among others. To observe a null phenotype for a PcG gene, one must remove its maternal as well as zygotic contribution to the embryo. Five members of the PcG group are compared here: Enhancer of Polycomb [E(Pc)], Additional sex combs (Asx), Posterior sex combs (Psc), Suppressor of zeste 2 [Su(z)2] and Polycomblike (Pcl). The yeast recombinase (FLP) system was used to induce mitotic recombination in the maternal germline. Mutant embryos were analyzed by staining with antibodies against six target genes of the PcG. The loss of the maternal component leads to enhanced homeotic phenotypes and to unique patterns of misexpression. E(Pc) and Su(z)2 mutations bad only subtle effects on the target genes, even when the maternal contributions were removed. Asx and Pcl mutants show derepression of the targets only in specific cell types. Psc shows unusual effects on two of the targets, Ultrabithorax and abdominal-A. These results show that the PcG genes do not act only in a common complex or pathway; they must have some independent functions.

Original languageEnglish (US)
Pages (from-to)231-243
Number of pages13
JournalGenetics
Volume140
Issue number1
StatePublished - 1995
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics

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