TY - JOUR
T1 - Comparison of pausing during transcription and replication
AU - Laflamme, Susan E.
AU - Kramer, Fred Russell
AU - Mills, Donald R.
N1 - Funding Information:
ACKNOWLEDGEMENTS We thank John Mack for the preparation of the illustrations. This work was supported by National Institutes of Health Grant GM-32044 and National Science Foundation Grant PCM-82-15902.
PY - 1985/12/9
Y1 - 1985/12/9
N2 - Pausing during the transcription of MDV-1 cDNA by Escherichia coli RNA polymerase was compared with pausing during the replication of MDV-1 RNA by Qβ replicase. MDV-1 RNA is able to form many strong hairpin structures, and Qβ replicase pauses after the synthesis of each [Mills et a1. (1978) Cell 15, 541-550]. Although the transcripts were virtually identical to MDV-1 RNA, the locations at which RNA polymerase paused were different and apparently were not related to sequences that can form hairpins. These results indicate that hairpin stability, per se, cannot be used to predict the occurrence of pausing during transcription.Four pauses that occur within a 5-nucleotide region were studied in detail. Insertions and deletions were made in the template DNA to determine the contribution made by the surrounding sequences to these pauses. The results indicate that some of the pauses require the presence of particular upstream sequences, while others are unaffected by the template modifications. Thus, there are at least two different transcriptional pausing mechanisms: one depends on the nature of upstream sequences, while the other is independent of upstream sequences.
AB - Pausing during the transcription of MDV-1 cDNA by Escherichia coli RNA polymerase was compared with pausing during the replication of MDV-1 RNA by Qβ replicase. MDV-1 RNA is able to form many strong hairpin structures, and Qβ replicase pauses after the synthesis of each [Mills et a1. (1978) Cell 15, 541-550]. Although the transcripts were virtually identical to MDV-1 RNA, the locations at which RNA polymerase paused were different and apparently were not related to sequences that can form hairpins. These results indicate that hairpin stability, per se, cannot be used to predict the occurrence of pausing during transcription.Four pauses that occur within a 5-nucleotide region were studied in detail. Insertions and deletions were made in the template DNA to determine the contribution made by the surrounding sequences to these pauses. The results indicate that some of the pauses require the presence of particular upstream sequences, while others are unaffected by the template modifications. Thus, there are at least two different transcriptional pausing mechanisms: one depends on the nature of upstream sequences, while the other is independent of upstream sequences.
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U2 - 10.1093/nar/13.23.8425
DO - 10.1093/nar/13.23.8425
M3 - Article
C2 - 3841202
AN - SCOPUS:0022422409
VL - 13
SP - 8425
EP - 8440
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 23
ER -