Comparison of recovery, inhibition and fixation of damage after heating and radiation

G. P. Raaphorst, E. I. Azzam

Research output: Contribution to journalArticlepeer-review

Abstract

1. 1.|Aphidicolin and β ara A, which are known inhibitors of polymerases α and β, can be used to evaluate the role of polymerases in recovery of radiation damage. 2. 2.|In this study the effect of these compounds was tested on recovery of potentially lethal damage after irradiation (X-PLD) in normal and transformed cells. In addition, recovery of potentially lethal damage and its inhibition after hyperthermia (H-PLD) were also examined and compared to radiation. 3. 3.|X-PLD recovery was inhibited to different degrees by β Ara A in a host of transformed and normal C3H 10T1/2 cells. Aphidicolin had no effect on X-PLD recovery. These data indicated that polymerase β and not polymerase α was involved in X-PLD recovery after irradiation. 4. 4.|H-PLD recovery after hyperthermia was not affected by β Ara A or aphidicolin indicating that H-PLD after hyperthermia was different than XPLD after X-irradiation.

Original languageEnglish (US)
Pages (from-to)19-24
Number of pages6
JournalJournal of Thermal Biology
Volume17
Issue number1
DOIs
StatePublished - Jan 1992
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • General Agricultural and Biological Sciences
  • Developmental Biology

Keywords

  • Hyperthermia
  • aphidicolin
  • polymerases α
  • radiation damage repair
  • β
  • βAra A

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