Comparison of the A-Cc curve fitting methods in determining maximum ribulose 1·5-bisphosphate carboxylase/oxygenase carboxylation rate, potential light saturated electron transport rate and leaf dark respiration

Zewei Miao, Ming Xu, Richard G. Lathrop, Yufei Wang

Research output: Contribution to journalArticlepeer-review

60 Scopus citations

Abstract

A review of the literature revealed that a variety of methods are currently used for fitting net assimilation of CO2-chloroplastic CO 2 concentration (A-Cc) curves, resulting in considerable differences in estimating the A-Cc parameters [including maximum ribulose 1·5-bisphosphate carboxylase/oxygenase (Rubisco) carboxylation rate (Vcmax), potential light saturated electron transport rate (Jmax), leaf dark respiration in the light (Rd), mesophyll conductance (gm) and triose-phosphate utilization (TPU)]. In this paper, we examined the impacts of fitting methods on the estimations of V cmax, Jmax, TPU, Rd and gm using grid search and non-linear fitting techniques. Our results suggested that the fitting methods significantly affected the predictions of Rubisco-limited (Ac), ribulose 1,5-bisphosphate-limited (Aj) and TPU-limited (Ap) curves and leaf photosynthesis velocities because of the inconsistent estimate of Vcmax, Jmax, TPU, R d and gm, but they barely influenced the Jmax : Vcmax, Vcmax : Rd and Jmax : TPU ratio. In terms of fitting accuracy, simplicity of fitting procedures and sample size requirement, we recommend to combine grid search and non-linear techniques to directly and simultaneously fit Vcmax, Jmax, TPU, Rd and gm with the whole A-Cc curve in contrast to the conventional method, which fits Vcmax, Rd or gm first and then solves for Vcmax, Jmax and/or TPU with Vcmax, Rd and/or gm held as constants.

Original languageEnglish (US)
Pages (from-to)109-122
Number of pages14
JournalPlant, Cell and Environment
Volume32
Issue number2
DOIs
StatePublished - Feb 2009

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

Keywords

  • A-C curve
  • Chloroplastic CO concentration
  • Grid search
  • Leaf photosynthesis
  • New Jersey Pinelands
  • Non-linear fitting

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