Competitive adsorption of albumin, fibronectin and collagen type I on different biomaterial surfaces: A QCM-D study

Helena Felgueiras, V. Migonney, S. Sommerfeld, N. S. Murthy, J. Kohn

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Scopus citations

Abstract

Adsorption of several proteins, individually and in a mixture, onto substrates was investigated. Five biomaterial surfaces were selected based on their functionality and/or hydrophilicity: Ti6Al4V, Ti6Al4V + poly(sodium styrene sulfonate) (poly(NaSS)), gold, poly(desamino tyrosyltyrosine ethyl ester carbonate) (poly(DTE)), polystyrene (PS). Their ability to interact with BSA, Fn and Col I was studied using a QCM-D technique. The presence of sulfonate groups from the poly(NaSS) coating significantly increased protein adsorption. When two proteins were pooled, in the majority of the cases, the first to adsorb onto the surface inhibited the adsorption of the second. One exception is the combination of Col I with Fn, which could be attributed to probable changes in the protein conformation. The advantage of these five surfaces in the biomedical field was confirmed by the ability of MC3T3-E1 osteoblastic cells to attach to the entire group.

Original languageEnglish (US)
Title of host publication13th Mediterranean Conference on Medical and Biological Engineering and Computing 2013 - MEDICON 2013
PublisherSpringer Verlag
Pages1597-1600
Number of pages4
ISBN (Print)9783319008455
DOIs
StatePublished - 2014
Event13th Mediterranean Conference on Medical and Biological Engineering and Computing 2013, MEDICON 2013 - Seville, Spain
Duration: Sep 25 2013Sep 28 2013

Publication series

NameIFMBE Proceedings
Volume41
ISSN (Print)1680-0737

Other

Other13th Mediterranean Conference on Medical and Biological Engineering and Computing 2013, MEDICON 2013
Country/TerritorySpain
CitySeville
Period9/25/139/28/13

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomedical Engineering

Keywords

  • Albumin
  • Biomaterials
  • Collagen I
  • Fibronectin
  • Quartz crystal microbalance

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