TY - JOUR
T1 - Complete nucleotide sequence of bean pod mottle virus RNA1
T2 - Sequence comparisons and evolutionary relationships to other comoviruses
AU - Di, Rong
AU - Hu, Chung Chi
AU - Ghabrial, Said A.
N1 - Funding Information:
We thank Anthony Clark for his helpful comments. This research was funded in part by a grant from The United Soybean Board ( project #8212) and is published as Journal Series Paper 98-12-59 of the Kentucky Agricultural Experiment Station.
PY - 1999
Y1 - 1999
N2 - The complete nucleotide sequence of bean pod mottle comovirus (BPMV) RNA1 was determined. It is 5983 nucleotides long, excluding the poly(A) tail, and encodes a polyprotein of 1850 amino acid (aa) residues. Multiple alignments of the deduced aa sequence of BPMV polyprotein with those of cowpea mosaic virus (CPMV), red clover mottle virus (RCMV) and cowpea severe mosaic virus (CPSMV) indicated that BPMV RNA1 encodes the predicted set of five mature proteins: the equivalent of CPMV 32K protease cofactor, 58K putative helicase, VPg, 24K protease and 87K putative RNA-dependent RNA polymerase. Of the four proposed cleavage sites in BPMV RNA1 polyprotein, the one at the 32K/58K site (Q/A) is distinct for BPMV polyprotein and those at the 58K/VPg and VPg/24K junctions (Q/S and Q/M, respectively) are identical in all four comovirus polyproteins. Sequence comparison and phylogenetic analysis revealed that BPMV RNA1 is more closely related to CPSMV than to CPMV or to RCMV.
AB - The complete nucleotide sequence of bean pod mottle comovirus (BPMV) RNA1 was determined. It is 5983 nucleotides long, excluding the poly(A) tail, and encodes a polyprotein of 1850 amino acid (aa) residues. Multiple alignments of the deduced aa sequence of BPMV polyprotein with those of cowpea mosaic virus (CPMV), red clover mottle virus (RCMV) and cowpea severe mosaic virus (CPSMV) indicated that BPMV RNA1 encodes the predicted set of five mature proteins: the equivalent of CPMV 32K protease cofactor, 58K putative helicase, VPg, 24K protease and 87K putative RNA-dependent RNA polymerase. Of the four proposed cleavage sites in BPMV RNA1 polyprotein, the one at the 32K/58K site (Q/A) is distinct for BPMV polyprotein and those at the 58K/VPg and VPg/24K junctions (Q/S and Q/M, respectively) are identical in all four comovirus polyproteins. Sequence comparison and phylogenetic analysis revealed that BPMV RNA1 is more closely related to CPSMV than to CPMV or to RCMV.
KW - Comovirus
KW - Genome organization
KW - Phylogenetic analysis
KW - Sequence comparison
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U2 - 10.1023/A:1008012502470
DO - 10.1023/A:1008012502470
M3 - Article
C2 - 10403699
AN - SCOPUS:0032971980
SN - 0920-8569
VL - 18
SP - 129
EP - 137
JO - Virus Genes
JF - Virus Genes
IS - 2
ER -