Complex formation between RAS and RAF and other protein kinases

Linda Van Aelst, Maureen Barr, Stevan Marcus, Anthony Polverino, Michael Wigler

Research output: Contribution to journalArticlepeer-review

588 Scopus citations


We used a Saccharomyces cerevisiae genetic system to detect the physical interaction of RAS and RAF oncoproteins. We also observed interaction between RAS and byr2, a protein kinase implicated as a mediator of the Schizosaccharomyces pombe ras1 protein. Interaction with RAS required only the N-terminal domains of RAF or byr2 and was disrupted by mutations in either the guanine nucleotide-binding or effector-loop domains of RAS. We observed interaction between MEK (a kinase that phosphorylates mitogen-activated protein kinases) and the catalytic domain of RAF. RAS and MEK also interacted but only when RAF was overexpressed.

Original languageEnglish (US)
Pages (from-to)6213-6217
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number13
StatePublished - Jul 1 1993
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General


  • Cancer
  • Protein-protein interactions
  • Signal transduction
  • Yeast


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