The protein concentration in biological samples is a crucial limiting factor for a successful analysis by electrophoresis. Many techniques have been adopted to increase protein concentrations, however, they are often insufficient and require special equipment or poisonous chemicals. Herein, we report a simple and efficient technique for concentrating dilute protein samples by absorbing proteins onto protein-blotting membrane strips. in this technique, blotting membrane strips were incubated in dilute protein solutions to capture proteins. For either sodium dodecyl sulphate-polyacrylamide gel electrophoresis or isoelectric focusing, the protein-absorbed membrane strips were directly loaded to the sample wells which contained a strong protein elution buffer and electrophoresis was performed under standard conditions.
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Organic Chemistry
- Sample preparation