Concentrating protein samples for sodium dodecyl sulphatepolyacrylamide gel eleccrophoresis and isoelectric focusing using protein-blotting membranes

Fang Ting Liang, David E. Granstrom, Yu Fang Shi

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The protein concentration in biological samples is a crucial limiting factor for a successful analysis by electrophoresis. Many techniques have been adopted to increase protein concentrations, however, they are often insufficient and require special equipment or poisonous chemicals. Herein, we report a simple and efficient technique for concentrating dilute protein samples by absorbing proteins onto protein-blotting membrane strips. in this technique, blotting membrane strips were incubated in dilute protein solutions to capture proteins. For either sodium dodecyl sulphate-polyacrylamide gel electrophoresis or isoelectric focusing, the protein-absorbed membrane strips were directly loaded to the sample wells which contained a strong protein elution buffer and electrophoresis was performed under standard conditions.

Original languageEnglish (US)
Pages (from-to)143-150
Number of pages8
JournalJournal of Chromatography A
Volume764
Issue number1
DOIs
StatePublished - Mar 7 1997
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Keywords

  • Membranes
  • Proteins
  • Sample preparation

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