Conformational polymorphisms of crna of T-cell-receptor genes as a clone-specific molecular marker for cutaneous lymphoma

Matthias Volkenandt, Ralf Wienecke, Olaf M. Koch, Jan Buer, Michael Probst, Jens Atzpodien, Tetsuro Horikoshi, Kathleen Danenberg, Peter Danenberg, Joseph R. Bertino

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

A novel molecular assay for the detection and characterization of monoclonal lymphoid populations in clinical specimens was developed. The assay is based on the principle that upon non-denaturing polyacrylamide gel electrophoresis RNA molecules separate into several metastable conformational forms. These conformational polymorphisms strictly depend on the nucleotide sequence of the individual molecule. Using DNA from formalin-fixed, paraffin-embedded tissue of patients with mycosis fungoides, highly variable junctional sequences of rearranged T-cell receptor gamma genes were amplified by polymerase chain reaction. Subsequently, the polymerase chain reactions products were transcribed into complementary RNA and analyzed by non-denaturing polyacrylamide gel electrophoresis. In clinical specimens with a monoclonal lymphoid population, a clone-specific pattern of bands was identified representing conformational polymorphisms of cRNA molecules of rearranged T-cell receptor gamma genes of the predominant lymphoid clone. Three biopsies from one patient taken from different sites of the body over 3 years yielded an identical pattern of bands. This methodology provides a novel and rapid tool for V the molecular identification and characterization of clonal lymphoid populations in clinical specimens. It is likely to be of special value for studies on the clonal evolution of lymphyloid disorders of the skin.

Original languageEnglish (US)
Pages (from-to)514-516
Number of pages3
JournalJournal of Investigative Dermatology
Volume101
Issue number4
DOIs
StatePublished - Oct 1993

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Keywords

  • lymphoma/polymerase chain reaction/clonality detection/T-cell-receptor genes

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