Constriction velocities of renal afferent and efferent arterioles of mice are not related to SMB expression

Andreas Patzak; Daria Petzhold; Thomas Wronski; Peter Martinka; Gopal J. Babu; Muthu Periasamy; Hannelore Haase; Ingo Morano

doi:10.1111/j.1523-1755.2005.00743.x

Constriction velocities of renal afferent and efferent arterioles of mice are not related to SMB expression

Andreas Patzak, Daria Petzhold, Thomas Wronski, Peter Martinka, Gopal J. Babu, Muthu Periasamy, Hannelore Haase, Ingo Morano

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Background. Constriction of renal arterioles contributes significantly to the control of perfusion and glomerular filtration. Afferent but not efferent arterioles express smooth muscle myosin heavy chain B (SMB) (with a 5′-insert of seven amino acids). The aim of the present study was to investigate (1) the constriction characteristics of afferent and efferent arterioles under physiologic load and (2) whether expression of SMB may causally contribute to these constriction characteristics. Methods. We compared constriction parameters [constriction amplitude, maximal rate of constriction velocity ("dc/dt_max"), and time to half-maximal constriction (t_1/2) of in vitro perfused renal afferent and efferent arterioles of wild-type (smb(+/+)] and homozygous SMB knockout [smb(-/-)] mice upon stimulation with angiotensin II (Ang II) (10^-8 mol/L) and potassium chloride (KCl) (100 mmol/L). SMB expression was investigated by double-labeling immunofluorescence. Results. Contraction amplitude and dc/dt_max of mouse afferent arterioles upon Ang II stimulation were significantly greater compared to efferent arterioles. However, constriction amplitudes, dc/dt_max, and t_1/2 of afferent as well as efferent arterioles upon Ang II stimulation were similar in smb(+/+) and smb(-/-) mice. Constriction amplitudes upon KCl stimulation of afferent arterioles were similar in both smb(+/+) and smb(-/-) mice. Furthermore, KCl-induced dc/dt_max and t_1/2 of afferent arterioles were similar in both smb(+/+) and smb(-/-) mice. SMB expression could be detected in afferent but not efferent arterioles in smb(+/+) mice. No SMB expression in either arteriole could be observed in smb(-/-) mice. Conclusion. Our results suggest that the presence of different alternatively 5′-spliced smooth muscle-myosin heavy chain (SM-MHC) isoforms does not dominate the different contractile features of physiologically loaded renal afferent or efferent arterioles.

Original language	English (US)
Pages (from-to)	2726-2734
Number of pages	9
Journal	Kidney International
Volume	68
Issue number	6
DOIs	https://doi.org/10.1111/j.1523-1755.2005.00743.x
State	Published - Dec 2005
Externally published	Yes

All Science Journal Classification (ASJC) codes

Nephrology

Keywords

Afferent arteriole
Efferent arteriole
Juxtaglomerular apparatus
Myosin heavy chains
Smooth muscle

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10.1111/j.1523-1755.2005.00743.x

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@article{d7e14da00a964746a3857a67315fe3c6,

title = "Constriction velocities of renal afferent and efferent arterioles of mice are not related to SMB expression",

abstract = "Background. Constriction of renal arterioles contributes significantly to the control of perfusion and glomerular filtration. Afferent but not efferent arterioles express smooth muscle myosin heavy chain B (SMB) (with a 5′-insert of seven amino acids). The aim of the present study was to investigate (1) the constriction characteristics of afferent and efferent arterioles under physiologic load and (2) whether expression of SMB may causally contribute to these constriction characteristics. Methods. We compared constriction parameters [constriction amplitude, maximal rate of constriction velocity ({"}dc/dtmax{"}), and time to half-maximal constriction (t1/2) of in vitro perfused renal afferent and efferent arterioles of wild-type (smb(+/+)] and homozygous SMB knockout [smb(-/-)] mice upon stimulation with angiotensin II (Ang II) (10-8 mol/L) and potassium chloride (KCl) (100 mmol/L). SMB expression was investigated by double-labeling immunofluorescence. Results. Contraction amplitude and dc/dtmax of mouse afferent arterioles upon Ang II stimulation were significantly greater compared to efferent arterioles. However, constriction amplitudes, dc/dtmax, and t1/2 of afferent as well as efferent arterioles upon Ang II stimulation were similar in smb(+/+) and smb(-/-) mice. Constriction amplitudes upon KCl stimulation of afferent arterioles were similar in both smb(+/+) and smb(-/-) mice. Furthermore, KCl-induced dc/dtmax and t1/2 of afferent arterioles were similar in both smb(+/+) and smb(-/-) mice. SMB expression could be detected in afferent but not efferent arterioles in smb(+/+) mice. No SMB expression in either arteriole could be observed in smb(-/-) mice. Conclusion. Our results suggest that the presence of different alternatively 5′-spliced smooth muscle-myosin heavy chain (SM-MHC) isoforms does not dominate the different contractile features of physiologically loaded renal afferent or efferent arterioles.",

keywords = "Afferent arteriole, Efferent arteriole, Juxtaglomerular apparatus, Myosin heavy chains, Smooth muscle",

author = "Andreas Patzak and Daria Petzhold and Thomas Wronski and Peter Martinka and Babu, {Gopal J.} and Muthu Periasamy and Hannelore Haase and Ingo Morano",

year = "2005",

month = dec,

doi = "10.1111/j.1523-1755.2005.00743.x",

language = "English (US)",

volume = "68",

pages = "2726--2734",

journal = "Kidney International",

issn = "0085-2538",

publisher = "Elsevier B.V.",

number = "6",

}

TY - JOUR

T1 - Constriction velocities of renal afferent and efferent arterioles of mice are not related to SMB expression

AU - Patzak, Andreas

AU - Petzhold, Daria

AU - Wronski, Thomas

AU - Martinka, Peter

AU - Babu, Gopal J.

AU - Periasamy, Muthu

AU - Haase, Hannelore

AU - Morano, Ingo

PY - 2005/12

Y1 - 2005/12

N2 - Background. Constriction of renal arterioles contributes significantly to the control of perfusion and glomerular filtration. Afferent but not efferent arterioles express smooth muscle myosin heavy chain B (SMB) (with a 5′-insert of seven amino acids). The aim of the present study was to investigate (1) the constriction characteristics of afferent and efferent arterioles under physiologic load and (2) whether expression of SMB may causally contribute to these constriction characteristics. Methods. We compared constriction parameters [constriction amplitude, maximal rate of constriction velocity ("dc/dtmax"), and time to half-maximal constriction (t1/2) of in vitro perfused renal afferent and efferent arterioles of wild-type (smb(+/+)] and homozygous SMB knockout [smb(-/-)] mice upon stimulation with angiotensin II (Ang II) (10-8 mol/L) and potassium chloride (KCl) (100 mmol/L). SMB expression was investigated by double-labeling immunofluorescence. Results. Contraction amplitude and dc/dtmax of mouse afferent arterioles upon Ang II stimulation were significantly greater compared to efferent arterioles. However, constriction amplitudes, dc/dtmax, and t1/2 of afferent as well as efferent arterioles upon Ang II stimulation were similar in smb(+/+) and smb(-/-) mice. Constriction amplitudes upon KCl stimulation of afferent arterioles were similar in both smb(+/+) and smb(-/-) mice. Furthermore, KCl-induced dc/dtmax and t1/2 of afferent arterioles were similar in both smb(+/+) and smb(-/-) mice. SMB expression could be detected in afferent but not efferent arterioles in smb(+/+) mice. No SMB expression in either arteriole could be observed in smb(-/-) mice. Conclusion. Our results suggest that the presence of different alternatively 5′-spliced smooth muscle-myosin heavy chain (SM-MHC) isoforms does not dominate the different contractile features of physiologically loaded renal afferent or efferent arterioles.

AB - Background. Constriction of renal arterioles contributes significantly to the control of perfusion and glomerular filtration. Afferent but not efferent arterioles express smooth muscle myosin heavy chain B (SMB) (with a 5′-insert of seven amino acids). The aim of the present study was to investigate (1) the constriction characteristics of afferent and efferent arterioles under physiologic load and (2) whether expression of SMB may causally contribute to these constriction characteristics. Methods. We compared constriction parameters [constriction amplitude, maximal rate of constriction velocity ("dc/dtmax"), and time to half-maximal constriction (t1/2) of in vitro perfused renal afferent and efferent arterioles of wild-type (smb(+/+)] and homozygous SMB knockout [smb(-/-)] mice upon stimulation with angiotensin II (Ang II) (10-8 mol/L) and potassium chloride (KCl) (100 mmol/L). SMB expression was investigated by double-labeling immunofluorescence. Results. Contraction amplitude and dc/dtmax of mouse afferent arterioles upon Ang II stimulation were significantly greater compared to efferent arterioles. However, constriction amplitudes, dc/dtmax, and t1/2 of afferent as well as efferent arterioles upon Ang II stimulation were similar in smb(+/+) and smb(-/-) mice. Constriction amplitudes upon KCl stimulation of afferent arterioles were similar in both smb(+/+) and smb(-/-) mice. Furthermore, KCl-induced dc/dtmax and t1/2 of afferent arterioles were similar in both smb(+/+) and smb(-/-) mice. SMB expression could be detected in afferent but not efferent arterioles in smb(+/+) mice. No SMB expression in either arteriole could be observed in smb(-/-) mice. Conclusion. Our results suggest that the presence of different alternatively 5′-spliced smooth muscle-myosin heavy chain (SM-MHC) isoforms does not dominate the different contractile features of physiologically loaded renal afferent or efferent arterioles.

KW - Afferent arteriole

KW - Efferent arteriole

KW - Juxtaglomerular apparatus

KW - Myosin heavy chains

KW - Smooth muscle

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U2 - 10.1111/j.1523-1755.2005.00743.x

DO - 10.1111/j.1523-1755.2005.00743.x

M3 - Article

C2 - 16316347

AN - SCOPUS:32844471451

SN - 0085-2538

VL - 68

SP - 2726

EP - 2734

JO - Kidney International

JF - Kidney International

IS - 6

ER -

Constriction velocities of renal afferent and efferent arterioles of mice are not related to SMB expression

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