Continuous microfluidic dna and protein trapping and concentration by balancing transverse electrokinetic forces

M. C. Morales, H. Lin, J. D. Zahn

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A continuous DNA and protein concentration method is demonstrated employing uniform direct-current (DC) fields applied transversally across a microchannel. Negatively charged samples are conjunctly infused in a converging Y-inlet with a receiving buffer of the same conductivity into a main daughter channel where coplanar electrodes running axially along the channel apply transverse fields. Depending on the buffer selection, samples could be concentrated by electrophoretic (EP) forces at the positive bias electrode or trapped at an equilibrium position within the channel by balancing the EP force with a drag force generated by an induced electroosmotic (EO) flow in the opposite direction.

Original languageEnglish (US)
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1451-1453
Number of pages3
StatePublished - 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: Oct 2 2011Oct 6 2011

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume3

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period10/2/1110/6/11

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Keywords

  • DNA concentration
  • Electroosmotic flow
  • Electrophoresis
  • Transverse fields

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