Cooperation between kinesin motors promotes spindle symmetry and chromosome organization in oocytes

Sarah J. Radford, Allysa Marie M. Go, Kim McKim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The oocyte spindle in most animal species is assembled in the absence of the microtubule-organizing centers called centrosomes. Without the organization provided by centrosomes, acentrosomal meiotic spindle organization may rely heavily on the bundling of microtubules by kinesin motor proteins. Indeed, the minus-end directed kinesin-14 NCD, and the plus-end directed kinesin-6 Subito are known to be required for oocyte spindle organization in Drosophila melanogaster. How multiple microtubulebundling kinesins interact to produce a functional acentrosomal spindle is not known. In addition, there have been few studies on the meiotic function of one of the most important microtubule-bundlers in mitotic cells, the kinesin-5 KLP61F. We have found that the kinesin-5 KLP61F is required for spindle and centromere symmetry in oocytes. The asymmetry observed in the absence of KLP61F depends on NCD, the kinesin-12 KLP54D, and the microcephaly protein ASP. In contrast, KLP61F and Subito work together in maintaining a bipolar spindle. We propose that the prominent central spindle, stabilized by Subito, provides the framework for the coordination of multiple microtubule-bundling activities. The activities of several proteins, including NCD, KLP54D, and ASP, generate asymmetries within the acentrosomal spindle, while KLP61F and Subito balance these forces, resulting in the capacity to accurately segregate chromosomes.

Original languageEnglish (US)
Pages (from-to)517-527
Number of pages11
JournalGenetics
Volume205
Issue number2
DOIs
StatePublished - Feb 1 2017

Fingerprint

Kinesin
Oocytes
Chromosomes
Microtubules
Centrosome
Microtubule-Organizing Center
Microcephaly
Spindle Apparatus
Proteins
Centromere
Drosophila melanogaster

All Science Journal Classification (ASJC) codes

  • Genetics

Keywords

  • Drosophila
  • Kinesin
  • Meiosis
  • Oocyte
  • Spindle

Cite this

Radford, Sarah J. ; Go, Allysa Marie M. ; McKim, Kim. / Cooperation between kinesin motors promotes spindle symmetry and chromosome organization in oocytes. In: Genetics. 2017 ; Vol. 205, No. 2. pp. 517-527.
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Cooperation between kinesin motors promotes spindle symmetry and chromosome organization in oocytes. / Radford, Sarah J.; Go, Allysa Marie M.; McKim, Kim.

In: Genetics, Vol. 205, No. 2, 01.02.2017, p. 517-527.

Research output: Contribution to journalArticle

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