Coordination of calcium signalling by endothelial-derived nitric oxide in the intact liver

Sandip Patel, Lawrence D. Robb-Gaspers, Kerri Anne Stellato, Mimi Shon, Andrew P. Thomas

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Calcium ions (Ca2+) and nitric oxide (NO) are key signalling molecules that are implicated in the regulation of numerous cellular processes. Here we show that, in the intact liver, stimulation of endothelial cells by bradykinin coordinates the propagation of vasopressin-dependent intercellular Ca2+ waves across hepatic plates, and markedly increases the frequency of Ca2+ oscillations in individual hepatocytes. Modulation of Ca2+ oscillations by bradykinin is lost following isolation of hepatocytes, but restored in co-cultures of hepatocytes and endothelial cells. The sensitizing effects of bradykinin are mimicked by NO donors and abrogated by NO inhibitors. Thus, crosstalk between NO and Ca2+ signalling pathways through the microvasculature is probably an important mechanism for the coordination of liver function and may have a function in other organs.

Original languageEnglish (US)
Pages (from-to)467-471
Number of pages5
JournalNature Cell Biology
Issue number8
StatePublished - Dec 1999


All Science Journal Classification (ASJC) codes

  • Cell Biology

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