Correlation between red blood cell deformability and changes in hemodynamic function

John Langenfeld, G. W. Machiedo, M. Lyons, B. F. Rush, G. Dikdan, T. W. Lysz

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Background. In sepsis red blood cells (RBCs) have been shown to be less deformable (i.e., more rigid) and have been implicated in decreasing nutrient blood supply and possibly leading to organ dysfunction. However, no studies have demonstrated an association between organ dysfunction and rigid RBCs. This study examined cardiovascular physiologic and histologic changes in two different models to determine whether a relationship may exist between RBC deformability and organ function. Methods. In the following two experiments, cardiac index (CI) was continuously measured, whereas both deformability index and histology were examined at the end of the experimental periods. The first experiment studied nonanesthetized, hydrated rats after a cecal ligation and puncture (CLP), a slow-developing means of inducing RBC rigidity. In a second experiment animals were anesthetized and received a 20% total blood volume transfusion of either diamide-treated (rigid) RBCs or normal RBCs. Results. CLP-treated animals' CI gradually decreased during 18 hours (232 ± 60 ml/min/kg to 123 ± 90 ml/min/kg; p = 0.05), with an increase in systemic vascular resistance (1459 ± 517 dyne · sec/cm5 · m2 to 2337 ± 1213 dyne · sec/cm5 · m2; p = 0.02). Diamide-treated animals had a rapid decrease in CI (86 ± 7.0 ml/min/kg to 58 ± 13 ml/min/kg; p = 0.05) and increase in SVR (2269 ± 373 dyne · sec/cm5 · m2 to 3897 ± 988 dyne · sec/cm5 · m2; p = 0.05) from baseline to 120 minutes after treatment respectively. The DI was significantly lower in both CLP and diamide groups (p < 0.03) when compared with control animals. Histologic evidence of subendocardial necrosis was shown in both the CLP- and Diamide- treated animals. Conclusions. These data suggest an association with RBC deformability and organ function in both septic and nonseptic animal models.

Original languageEnglish (US)
Pages (from-to)859-867
Number of pages9
JournalSurgery
Volume116
Issue number5
StatePublished - Jan 1 1994

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Diamide
Erythrocytes
Hemodynamics
Punctures
Ligation
Blood Volume
Blood Transfusion
Vascular Resistance
Sepsis
Histology
Necrosis
Animal Models
Food

All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

Langenfeld, J., Machiedo, G. W., Lyons, M., Rush, B. F., Dikdan, G., & Lysz, T. W. (1994). Correlation between red blood cell deformability and changes in hemodynamic function. Surgery, 116(5), 859-867.
Langenfeld, John ; Machiedo, G. W. ; Lyons, M. ; Rush, B. F. ; Dikdan, G. ; Lysz, T. W. / Correlation between red blood cell deformability and changes in hemodynamic function. In: Surgery. 1994 ; Vol. 116, No. 5. pp. 859-867.
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abstract = "Background. In sepsis red blood cells (RBCs) have been shown to be less deformable (i.e., more rigid) and have been implicated in decreasing nutrient blood supply and possibly leading to organ dysfunction. However, no studies have demonstrated an association between organ dysfunction and rigid RBCs. This study examined cardiovascular physiologic and histologic changes in two different models to determine whether a relationship may exist between RBC deformability and organ function. Methods. In the following two experiments, cardiac index (CI) was continuously measured, whereas both deformability index and histology were examined at the end of the experimental periods. The first experiment studied nonanesthetized, hydrated rats after a cecal ligation and puncture (CLP), a slow-developing means of inducing RBC rigidity. In a second experiment animals were anesthetized and received a 20{\%} total blood volume transfusion of either diamide-treated (rigid) RBCs or normal RBCs. Results. CLP-treated animals' CI gradually decreased during 18 hours (232 ± 60 ml/min/kg to 123 ± 90 ml/min/kg; p = 0.05), with an increase in systemic vascular resistance (1459 ± 517 dyne · sec/cm5 · m2 to 2337 ± 1213 dyne · sec/cm5 · m2; p = 0.02). Diamide-treated animals had a rapid decrease in CI (86 ± 7.0 ml/min/kg to 58 ± 13 ml/min/kg; p = 0.05) and increase in SVR (2269 ± 373 dyne · sec/cm5 · m2 to 3897 ± 988 dyne · sec/cm5 · m2; p = 0.05) from baseline to 120 minutes after treatment respectively. The DI was significantly lower in both CLP and diamide groups (p < 0.03) when compared with control animals. Histologic evidence of subendocardial necrosis was shown in both the CLP- and Diamide- treated animals. Conclusions. These data suggest an association with RBC deformability and organ function in both septic and nonseptic animal models.",
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Langenfeld, J, Machiedo, GW, Lyons, M, Rush, BF, Dikdan, G & Lysz, TW 1994, 'Correlation between red blood cell deformability and changes in hemodynamic function', Surgery, vol. 116, no. 5, pp. 859-867.

Correlation between red blood cell deformability and changes in hemodynamic function. / Langenfeld, John; Machiedo, G. W.; Lyons, M.; Rush, B. F.; Dikdan, G.; Lysz, T. W.

In: Surgery, Vol. 116, No. 5, 01.01.1994, p. 859-867.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Correlation between red blood cell deformability and changes in hemodynamic function

AU - Langenfeld, John

AU - Machiedo, G. W.

AU - Lyons, M.

AU - Rush, B. F.

AU - Dikdan, G.

AU - Lysz, T. W.

PY - 1994/1/1

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N2 - Background. In sepsis red blood cells (RBCs) have been shown to be less deformable (i.e., more rigid) and have been implicated in decreasing nutrient blood supply and possibly leading to organ dysfunction. However, no studies have demonstrated an association between organ dysfunction and rigid RBCs. This study examined cardiovascular physiologic and histologic changes in two different models to determine whether a relationship may exist between RBC deformability and organ function. Methods. In the following two experiments, cardiac index (CI) was continuously measured, whereas both deformability index and histology were examined at the end of the experimental periods. The first experiment studied nonanesthetized, hydrated rats after a cecal ligation and puncture (CLP), a slow-developing means of inducing RBC rigidity. In a second experiment animals were anesthetized and received a 20% total blood volume transfusion of either diamide-treated (rigid) RBCs or normal RBCs. Results. CLP-treated animals' CI gradually decreased during 18 hours (232 ± 60 ml/min/kg to 123 ± 90 ml/min/kg; p = 0.05), with an increase in systemic vascular resistance (1459 ± 517 dyne · sec/cm5 · m2 to 2337 ± 1213 dyne · sec/cm5 · m2; p = 0.02). Diamide-treated animals had a rapid decrease in CI (86 ± 7.0 ml/min/kg to 58 ± 13 ml/min/kg; p = 0.05) and increase in SVR (2269 ± 373 dyne · sec/cm5 · m2 to 3897 ± 988 dyne · sec/cm5 · m2; p = 0.05) from baseline to 120 minutes after treatment respectively. The DI was significantly lower in both CLP and diamide groups (p < 0.03) when compared with control animals. Histologic evidence of subendocardial necrosis was shown in both the CLP- and Diamide- treated animals. Conclusions. These data suggest an association with RBC deformability and organ function in both septic and nonseptic animal models.

AB - Background. In sepsis red blood cells (RBCs) have been shown to be less deformable (i.e., more rigid) and have been implicated in decreasing nutrient blood supply and possibly leading to organ dysfunction. However, no studies have demonstrated an association between organ dysfunction and rigid RBCs. This study examined cardiovascular physiologic and histologic changes in two different models to determine whether a relationship may exist between RBC deformability and organ function. Methods. In the following two experiments, cardiac index (CI) was continuously measured, whereas both deformability index and histology were examined at the end of the experimental periods. The first experiment studied nonanesthetized, hydrated rats after a cecal ligation and puncture (CLP), a slow-developing means of inducing RBC rigidity. In a second experiment animals were anesthetized and received a 20% total blood volume transfusion of either diamide-treated (rigid) RBCs or normal RBCs. Results. CLP-treated animals' CI gradually decreased during 18 hours (232 ± 60 ml/min/kg to 123 ± 90 ml/min/kg; p = 0.05), with an increase in systemic vascular resistance (1459 ± 517 dyne · sec/cm5 · m2 to 2337 ± 1213 dyne · sec/cm5 · m2; p = 0.02). Diamide-treated animals had a rapid decrease in CI (86 ± 7.0 ml/min/kg to 58 ± 13 ml/min/kg; p = 0.05) and increase in SVR (2269 ± 373 dyne · sec/cm5 · m2 to 3897 ± 988 dyne · sec/cm5 · m2; p = 0.05) from baseline to 120 minutes after treatment respectively. The DI was significantly lower in both CLP and diamide groups (p < 0.03) when compared with control animals. Histologic evidence of subendocardial necrosis was shown in both the CLP- and Diamide- treated animals. Conclusions. These data suggest an association with RBC deformability and organ function in both septic and nonseptic animal models.

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Langenfeld J, Machiedo GW, Lyons M, Rush BF, Dikdan G, Lysz TW. Correlation between red blood cell deformability and changes in hemodynamic function. Surgery. 1994 Jan 1;116(5):859-867.