TY - JOUR
T1 - Correlative Imaging of Trace Elements and Intact Molecular Species in a Single-Tissue Sample at the 50 μm Scale
AU - de Jesus, Janella Marie
AU - Costa, Catia
AU - Burton, Amy
AU - Palitsin, Vladimir
AU - Webb, Roger
AU - Taylor, Adam
AU - Nikula, Chelsea
AU - Dexter, Alex
AU - Kaya, Firat
AU - Chambers, Mark
AU - Dartois, Veronique
AU - Goodwin, Richard J.A.
AU - Bunch, Josephine
AU - Bailey, Melanie J.
N1 - Funding Information:
This study was funded by an EPSRC strategic equipment award (EP/P001440/1) and an EPSRC sponsored fellowship (EP/R031118/1), the Surrey-NPL studentship fund. Beam time was provided by EPSRC National Research Facility UKNIBC NS/A000059/1 and RADIATE (Horizon 2020, grant agreement No 824096, proposal 19001813-ST). Special thanks to John Swales from AstraZeneca for providing tissue homogenates. The authors acknowledge R.T. Steven from NPL for experimental support. The authors thank Laura E Via and Danielle Weiner from the NIAID-NIH for providing infected lung tissues.
Publisher Copyright:
© 2021 The Authors. Published by American Chemical Society
PY - 2021/10/12
Y1 - 2021/10/12
N2 - Elemental and molecular imaging play a crucial role in understanding disease pathogenesis. To accurately correlate elemental and molecular markers, it is desirable to perform sequential elemental and molecular imaging on a single-tissue section. However, very little is known about the impact of performing these measurements in sequence. In this work, we highlight some of the challenges and successes associated with performing elemental mapping in sequence with mass spectrometry imaging. Specifically, the feasibility of molecular mapping using the mass spectrometry imaging (MSI) techniques matrix-assisted laser desorption ionization (MALDI) and desorption electrospray ionization (DESI) in sequence with the elemental mapping technique particle-induced X-ray emission (PIXE) is explored. Challenges for integration include substrate compatibility, as well as delocalization and spectral changes. We demonstrate that while sequential imaging comes with some compromises, sequential DESI-PIXE imaging is sufficient to correlate sulfur, iron, and lipid markers in a single tissue section at the 50 μm scale.
AB - Elemental and molecular imaging play a crucial role in understanding disease pathogenesis. To accurately correlate elemental and molecular markers, it is desirable to perform sequential elemental and molecular imaging on a single-tissue section. However, very little is known about the impact of performing these measurements in sequence. In this work, we highlight some of the challenges and successes associated with performing elemental mapping in sequence with mass spectrometry imaging. Specifically, the feasibility of molecular mapping using the mass spectrometry imaging (MSI) techniques matrix-assisted laser desorption ionization (MALDI) and desorption electrospray ionization (DESI) in sequence with the elemental mapping technique particle-induced X-ray emission (PIXE) is explored. Challenges for integration include substrate compatibility, as well as delocalization and spectral changes. We demonstrate that while sequential imaging comes with some compromises, sequential DESI-PIXE imaging is sufficient to correlate sulfur, iron, and lipid markers in a single tissue section at the 50 μm scale.
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U2 - 10.1021/acs.analchem.1c01927
DO - 10.1021/acs.analchem.1c01927
M3 - Article
AN - SCOPUS:85117238276
VL - 93
SP - 13450
EP - 13458
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 40
ER -