Cosecretion of protease inhibitor stabilizes antibodies produced by plant roots

Slavko Komarnytsky, Nikolai Borisjuk, Nir Yakoby, Alison Garvey, Ilya Raskin

Research output: Contribution to journalArticlepeer-review

79 Scopus citations

Abstract

A plant-based system for continuous production of monoclonal antibodies based on the secretion of immunoglobulin complexes from plant roots into a hydroponic medium (rhizosecretion) was engineered to produce high levels of single-chain and full-size immunoglobulins. Replacing the original signal peptides of monoclonal antibodies with a plant-derived calreticulin signal increased the levels of antibody yield 2-fold. Cosecretion of Bowman-Birk Ser protease inhibitor reduced degradation of the immunoglobulin complexes in the default secretion pathway and further increased antibody production to 36.4 μg/g root dry weight per day for single-chain IgG1 and 21.8 μg/g root dry weight per day for full-size IgG4 antibodies. These results suggest that constitutive cosecretion of a protease inhibitor combined with the use of the plant signal peptide and the antibiotic marker-free transformation system offers a novel strategy to achieve high yields of complex therapeutic proteins secreted from plant roots.

Original languageEnglish (US)
Pages (from-to)1185-1193
Number of pages9
JournalPlant physiology
Volume141
Issue number4
DOIs
StatePublished - Aug 2006

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

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