Counterimmunoelectrophoresis for detection of human serum antibody to HTLV-III

Martin J. Blaser; David L. Cohn; Henry J. Cody; Kent A. Penley; Franklyn N. Judson; W. Carl Saxinger; Stanley H. Weiss

doi:10.1016/0022-1759(86)90477-1

Counterimmunoelectrophoresis for detection of human serum antibody to HTLV-III

Martin J. Blaser, David L. Cohn, Henry J. Cody, Kent A. Penley, Franklyn N. Judson, W. Carl Saxinger, Stanley H. Weiss

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

We examined the usefulness of a counterimmunoelectrophoresis (CIE) technique for detecting antibodies to HTLV-III using sera that previously had been assessed for antibodies to HTLV-III by the standard enzyme-linked immunosorbent assay (ELISA). We selected a subset of 53 sera from patients with the acquired immune deficiency syndrome (AIDS) or the generalized lymphadenopathy syndrome (GLS) in which 81.1% were initially ELISA-positive, and 96.2% were positive by Western blot technique. In our standard HTLV-III CIE technique, 58.5% were positive and repeat testing increased the yield to 67.9%. Varying several parameters of the standard CIE assay did not improve sensitivity. We also studied 20 ELISA-negative and 10 ELISA-borderline sera from normal controls; all were negative by CIE. These results indicate that CIE may be used for detection of human serum antibodies to HTLV-III, but that the present assay was less sensitive than the HTLV-III ELISA.

Original language	English (US)
Pages (from-to)	181-186
Number of pages	6
Journal	Journal of Immunological Methods
Volume	91
Issue number	2
DOIs	https://doi.org/10.1016/0022-1759(86)90477-1
State	Published - Jul 24 1986
Externally published	Yes

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Immunology

Keywords

AIDS
Antibody detection
Counterimmunoelectrophoresis
ELISA
HTLV-III
Western blotting

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title = "Counterimmunoelectrophoresis for detection of human serum antibody to HTLV-III",

abstract = "We examined the usefulness of a counterimmunoelectrophoresis (CIE) technique for detecting antibodies to HTLV-III using sera that previously had been assessed for antibodies to HTLV-III by the standard enzyme-linked immunosorbent assay (ELISA). We selected a subset of 53 sera from patients with the acquired immune deficiency syndrome (AIDS) or the generalized lymphadenopathy syndrome (GLS) in which 81.1% were initially ELISA-positive, and 96.2% were positive by Western blot technique. In our standard HTLV-III CIE technique, 58.5% were positive and repeat testing increased the yield to 67.9%. Varying several parameters of the standard CIE assay did not improve sensitivity. We also studied 20 ELISA-negative and 10 ELISA-borderline sera from normal controls; all were negative by CIE. These results indicate that CIE may be used for detection of human serum antibodies to HTLV-III, but that the present assay was less sensitive than the HTLV-III ELISA.",

keywords = "AIDS, Antibody detection, Counterimmunoelectrophoresis, ELISA, HTLV-III, Western blotting",

author = "Blaser, {Martin J.} and Cohn, {David L.} and Cody, {Henry J.} and Penley, {Kent A.} and Judson, {Franklyn N.} and {Carl Saxinger}, W. and Weiss, {Stanley H.}",

note = "Funding Information: Supported by Grant R01 HL-32434-01 from the National Heart, Lung and Blood Institute and by the Medical Research Service of the Veterans Administration. We thank Drs. Prem S. Sarin and Robert C. Gallo for their gift of inactivated HTLV-III virus and assistance in establishing these studies.",

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doi = "10.1016/0022-1759(86)90477-1",

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pages = "181--186",

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T1 - Counterimmunoelectrophoresis for detection of human serum antibody to HTLV-III

AU - Blaser, Martin J.

AU - Cohn, David L.

AU - Cody, Henry J.

AU - Penley, Kent A.

AU - Judson, Franklyn N.

AU - Carl Saxinger, W.

AU - Weiss, Stanley H.

N1 - Funding Information: Supported by Grant R01 HL-32434-01 from the National Heart, Lung and Blood Institute and by the Medical Research Service of the Veterans Administration. We thank Drs. Prem S. Sarin and Robert C. Gallo for their gift of inactivated HTLV-III virus and assistance in establishing these studies.

PY - 1986/7/24

Y1 - 1986/7/24

N2 - We examined the usefulness of a counterimmunoelectrophoresis (CIE) technique for detecting antibodies to HTLV-III using sera that previously had been assessed for antibodies to HTLV-III by the standard enzyme-linked immunosorbent assay (ELISA). We selected a subset of 53 sera from patients with the acquired immune deficiency syndrome (AIDS) or the generalized lymphadenopathy syndrome (GLS) in which 81.1% were initially ELISA-positive, and 96.2% were positive by Western blot technique. In our standard HTLV-III CIE technique, 58.5% were positive and repeat testing increased the yield to 67.9%. Varying several parameters of the standard CIE assay did not improve sensitivity. We also studied 20 ELISA-negative and 10 ELISA-borderline sera from normal controls; all were negative by CIE. These results indicate that CIE may be used for detection of human serum antibodies to HTLV-III, but that the present assay was less sensitive than the HTLV-III ELISA.

AB - We examined the usefulness of a counterimmunoelectrophoresis (CIE) technique for detecting antibodies to HTLV-III using sera that previously had been assessed for antibodies to HTLV-III by the standard enzyme-linked immunosorbent assay (ELISA). We selected a subset of 53 sera from patients with the acquired immune deficiency syndrome (AIDS) or the generalized lymphadenopathy syndrome (GLS) in which 81.1% were initially ELISA-positive, and 96.2% were positive by Western blot technique. In our standard HTLV-III CIE technique, 58.5% were positive and repeat testing increased the yield to 67.9%. Varying several parameters of the standard CIE assay did not improve sensitivity. We also studied 20 ELISA-negative and 10 ELISA-borderline sera from normal controls; all were negative by CIE. These results indicate that CIE may be used for detection of human serum antibodies to HTLV-III, but that the present assay was less sensitive than the HTLV-III ELISA.

KW - AIDS

KW - Antibody detection

KW - Counterimmunoelectrophoresis

KW - ELISA

KW - HTLV-III

KW - Western blotting

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Counterimmunoelectrophoresis for detection of human serum antibody to HTLV-III

Abstract

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