CREG inhibits migration of human vascular smooth muscle cells by mediating IGF-II endocytosis

Yaling Han, Jifu Cui, Jie Tao, Liang Guo, Peng Guo, Mingyu Sun, Jian Kang, Xiaolin Zhang, Chenghui Yan, Shaohua Li

Research output: Contribution to journalArticle

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Abstract

We previously determined that the cellular repressor of E1A-stimulated genes, (CREG) plays a role in the maintenance of the mature phenotype of vascular smooth muscle cells (SMCs). This study aimed to identify the role of CREG in modulating the migration of SMCs. Recombinant virus-mediated CREG expression inhibited the cellular migration of cultured SMCs associated with down-regulated activity of matrix metalloproteinase-9 (MMP-9). In contrast, CREG knockdown via the retroviral transfer of short hairpin RNAs promoted cellular migration. Enzyme-linked immunosorbent assay and endocytosis analysis revealed that CREG knockdown attenuated the internalization and increased secretion of insulin-like growth factor (IGF)-II. Western blot analysis demonstrated that both phosphoinositide 3-kinase (PI3K) and phosphatase Akt were enhanced in CREG knockdown SMCs. Furthermore, the effect of CREG knockdown on SMC migration was abrogated in a dose-dependent manner by the addition of either IGF-II neutralizing antibody or the PI3K inhibitor, LY294002. These results indicate that the CREG knockdown-mediated increase in IGF-II secretion promoted cellular migration in SMCs via the PI3K/Akt signal pathway. Additionally, blockage of IGF-II binding to the mannose-6-phosphate/IGF-II receptor (M6P/IGF2R) by IGF2R antibody or recombinant IGF2R fragment attenuated the endocytosis of IGF-II in cells overexpressing CREG. This indicates that M6P/IGF2R is involved in the regulation of CREG-mediated IGF-II endocytosis. In summary, these data demonstrate for the first time that CREG plays a critical role in the inhibition of SMC migration, as well as maintaining SMCs in a mature phenotype. These results may provide a new therapeutic target for vascular disease associated with neointimal hyperplasia.

Original languageEnglish (US)
Pages (from-to)3301-3311
Number of pages11
JournalExperimental cell research
Volume315
Issue number19
DOIs
StatePublished - Nov 15 2009

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Insulin-Like Growth Factor II
Endocytosis
Vascular Smooth Muscle
Smooth Muscle Myocytes
Gene Knockdown Techniques
Genes
1-Phosphatidylinositol 4-Kinase
Cell Movement
IGF Type 2 Receptor
Phenotype
2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
Matrix Metalloproteinase 9
Neutralizing Antibodies
Vascular Diseases
Small Interfering RNA
Hyperplasia
Signal Transduction
Western Blotting
Enzyme-Linked Immunosorbent Assay
Maintenance

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Han, Yaling ; Cui, Jifu ; Tao, Jie ; Guo, Liang ; Guo, Peng ; Sun, Mingyu ; Kang, Jian ; Zhang, Xiaolin ; Yan, Chenghui ; Li, Shaohua. / CREG inhibits migration of human vascular smooth muscle cells by mediating IGF-II endocytosis. In: Experimental cell research. 2009 ; Vol. 315, No. 19. pp. 3301-3311.
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abstract = "We previously determined that the cellular repressor of E1A-stimulated genes, (CREG) plays a role in the maintenance of the mature phenotype of vascular smooth muscle cells (SMCs). This study aimed to identify the role of CREG in modulating the migration of SMCs. Recombinant virus-mediated CREG expression inhibited the cellular migration of cultured SMCs associated with down-regulated activity of matrix metalloproteinase-9 (MMP-9). In contrast, CREG knockdown via the retroviral transfer of short hairpin RNAs promoted cellular migration. Enzyme-linked immunosorbent assay and endocytosis analysis revealed that CREG knockdown attenuated the internalization and increased secretion of insulin-like growth factor (IGF)-II. Western blot analysis demonstrated that both phosphoinositide 3-kinase (PI3K) and phosphatase Akt were enhanced in CREG knockdown SMCs. Furthermore, the effect of CREG knockdown on SMC migration was abrogated in a dose-dependent manner by the addition of either IGF-II neutralizing antibody or the PI3K inhibitor, LY294002. These results indicate that the CREG knockdown-mediated increase in IGF-II secretion promoted cellular migration in SMCs via the PI3K/Akt signal pathway. Additionally, blockage of IGF-II binding to the mannose-6-phosphate/IGF-II receptor (M6P/IGF2R) by IGF2R antibody or recombinant IGF2R fragment attenuated the endocytosis of IGF-II in cells overexpressing CREG. This indicates that M6P/IGF2R is involved in the regulation of CREG-mediated IGF-II endocytosis. In summary, these data demonstrate for the first time that CREG plays a critical role in the inhibition of SMC migration, as well as maintaining SMCs in a mature phenotype. These results may provide a new therapeutic target for vascular disease associated with neointimal hyperplasia.",
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Han, Y, Cui, J, Tao, J, Guo, L, Guo, P, Sun, M, Kang, J, Zhang, X, Yan, C & Li, S 2009, 'CREG inhibits migration of human vascular smooth muscle cells by mediating IGF-II endocytosis', Experimental cell research, vol. 315, no. 19, pp. 3301-3311. https://doi.org/10.1016/j.yexcr.2009.09.013

CREG inhibits migration of human vascular smooth muscle cells by mediating IGF-II endocytosis. / Han, Yaling; Cui, Jifu; Tao, Jie; Guo, Liang; Guo, Peng; Sun, Mingyu; Kang, Jian; Zhang, Xiaolin; Yan, Chenghui; Li, Shaohua.

In: Experimental cell research, Vol. 315, No. 19, 15.11.2009, p. 3301-3311.

Research output: Contribution to journalArticle

TY - JOUR

T1 - CREG inhibits migration of human vascular smooth muscle cells by mediating IGF-II endocytosis

AU - Han, Yaling

AU - Cui, Jifu

AU - Tao, Jie

AU - Guo, Liang

AU - Guo, Peng

AU - Sun, Mingyu

AU - Kang, Jian

AU - Zhang, Xiaolin

AU - Yan, Chenghui

AU - Li, Shaohua

PY - 2009/11/15

Y1 - 2009/11/15

N2 - We previously determined that the cellular repressor of E1A-stimulated genes, (CREG) plays a role in the maintenance of the mature phenotype of vascular smooth muscle cells (SMCs). This study aimed to identify the role of CREG in modulating the migration of SMCs. Recombinant virus-mediated CREG expression inhibited the cellular migration of cultured SMCs associated with down-regulated activity of matrix metalloproteinase-9 (MMP-9). In contrast, CREG knockdown via the retroviral transfer of short hairpin RNAs promoted cellular migration. Enzyme-linked immunosorbent assay and endocytosis analysis revealed that CREG knockdown attenuated the internalization and increased secretion of insulin-like growth factor (IGF)-II. Western blot analysis demonstrated that both phosphoinositide 3-kinase (PI3K) and phosphatase Akt were enhanced in CREG knockdown SMCs. Furthermore, the effect of CREG knockdown on SMC migration was abrogated in a dose-dependent manner by the addition of either IGF-II neutralizing antibody or the PI3K inhibitor, LY294002. These results indicate that the CREG knockdown-mediated increase in IGF-II secretion promoted cellular migration in SMCs via the PI3K/Akt signal pathway. Additionally, blockage of IGF-II binding to the mannose-6-phosphate/IGF-II receptor (M6P/IGF2R) by IGF2R antibody or recombinant IGF2R fragment attenuated the endocytosis of IGF-II in cells overexpressing CREG. This indicates that M6P/IGF2R is involved in the regulation of CREG-mediated IGF-II endocytosis. In summary, these data demonstrate for the first time that CREG plays a critical role in the inhibition of SMC migration, as well as maintaining SMCs in a mature phenotype. These results may provide a new therapeutic target for vascular disease associated with neointimal hyperplasia.

AB - We previously determined that the cellular repressor of E1A-stimulated genes, (CREG) plays a role in the maintenance of the mature phenotype of vascular smooth muscle cells (SMCs). This study aimed to identify the role of CREG in modulating the migration of SMCs. Recombinant virus-mediated CREG expression inhibited the cellular migration of cultured SMCs associated with down-regulated activity of matrix metalloproteinase-9 (MMP-9). In contrast, CREG knockdown via the retroviral transfer of short hairpin RNAs promoted cellular migration. Enzyme-linked immunosorbent assay and endocytosis analysis revealed that CREG knockdown attenuated the internalization and increased secretion of insulin-like growth factor (IGF)-II. Western blot analysis demonstrated that both phosphoinositide 3-kinase (PI3K) and phosphatase Akt were enhanced in CREG knockdown SMCs. Furthermore, the effect of CREG knockdown on SMC migration was abrogated in a dose-dependent manner by the addition of either IGF-II neutralizing antibody or the PI3K inhibitor, LY294002. These results indicate that the CREG knockdown-mediated increase in IGF-II secretion promoted cellular migration in SMCs via the PI3K/Akt signal pathway. Additionally, blockage of IGF-II binding to the mannose-6-phosphate/IGF-II receptor (M6P/IGF2R) by IGF2R antibody or recombinant IGF2R fragment attenuated the endocytosis of IGF-II in cells overexpressing CREG. This indicates that M6P/IGF2R is involved in the regulation of CREG-mediated IGF-II endocytosis. In summary, these data demonstrate for the first time that CREG plays a critical role in the inhibition of SMC migration, as well as maintaining SMCs in a mature phenotype. These results may provide a new therapeutic target for vascular disease associated with neointimal hyperplasia.

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U2 - 10.1016/j.yexcr.2009.09.013

DO - 10.1016/j.yexcr.2009.09.013

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