Cross-species infectivity of H3N8 influenza virus in an experimental infection in swine

Alicia Solórzano, Emanuela Foni, Lorena Córdoba, Massimiliano Baratelli, Elisabetta Razzuoli, Dania Bilato, María Ángeles Martín del Burgo, David S. Perlin, Jorge Martínez, Pamela Martínez-Orellana, Lorenzo Fraile, Chiara Chiapponi, Massimo Amadori, Gustavo del Real, María Montoya

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Avian influenza A viruses have gained increasing attention due to their ability to cross the species barrier and cause severe disease in humans and other mammal species as pigs. H3 and particularly H3N8 viruses, are highly adaptive since they are found in multiple avian and mammal hosts. H3N8 viruses have not been isolated yet from humans; however, a recent report showed that equine influenza A viruses (IAVs) can be isolated from pigs, although an established infection has not been observed thus far in this host. To gain insight into the possibility of H3N8 avian IAVs to cross the species barrier into pigs, in vitro experiments and an experimental infection in pigs with four H3N8 viruses from different origins (equine, canine, avian, and seal) were performed. As a positive control, an H3N2 swine influenza virus A was used. Although equine and canine viruses hardly replicated in the respiratory systems of pigs, avian and seal viruses replicated substantially and caused detectable lesions in inoculated pigs without previous adaptation. Interestingly, antibodies against hemagglutinin could not be detected after infection by hemagglutination inhibition (HAI) test with avian and seal viruses. This phenomenon was observed not only in pigs but also in mice immunized with the same virus strains. Our data indicated that H3N8 IAVs from wild aquatic birds have the potential to cross the species barrier and establish successful infections in pigs that might spread unnoticed using the HAI test as diagnostic tool.

Original languageEnglish (US)
Pages (from-to)11190-11202
Number of pages13
JournalJournal of virology
Volume89
Issue number22
DOIs
StatePublished - Jan 1 2015

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H3N8 Subtype Influenza A Virus
Orthomyxoviridae
Influenza A virus
Swine
pathogenicity
swine
Infection
infection
seals
viruses
hemagglutination inhibition test
Horses
Viruses
Hemagglutination Inhibition Tests
Influenza in Birds
Canidae
Mammals
mammals
equine influenza
horses

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Solórzano, A., Foni, E., Córdoba, L., Baratelli, M., Razzuoli, E., Bilato, D., ... Montoya, M. (2015). Cross-species infectivity of H3N8 influenza virus in an experimental infection in swine. Journal of virology, 89(22), 11190-11202. https://doi.org/10.1128/JVI.01509-15
Solórzano, Alicia ; Foni, Emanuela ; Córdoba, Lorena ; Baratelli, Massimiliano ; Razzuoli, Elisabetta ; Bilato, Dania ; del Burgo, María Ángeles Martín ; Perlin, David S. ; Martínez, Jorge ; Martínez-Orellana, Pamela ; Fraile, Lorenzo ; Chiapponi, Chiara ; Amadori, Massimo ; Real, Gustavo del ; Montoya, María. / Cross-species infectivity of H3N8 influenza virus in an experimental infection in swine. In: Journal of virology. 2015 ; Vol. 89, No. 22. pp. 11190-11202.
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Solórzano, A, Foni, E, Córdoba, L, Baratelli, M, Razzuoli, E, Bilato, D, del Burgo, MÁM, Perlin, DS, Martínez, J, Martínez-Orellana, P, Fraile, L, Chiapponi, C, Amadori, M, Real, GD & Montoya, M 2015, 'Cross-species infectivity of H3N8 influenza virus in an experimental infection in swine', Journal of virology, vol. 89, no. 22, pp. 11190-11202. https://doi.org/10.1128/JVI.01509-15

Cross-species infectivity of H3N8 influenza virus in an experimental infection in swine. / Solórzano, Alicia; Foni, Emanuela; Córdoba, Lorena; Baratelli, Massimiliano; Razzuoli, Elisabetta; Bilato, Dania; del Burgo, María Ángeles Martín; Perlin, David S.; Martínez, Jorge; Martínez-Orellana, Pamela; Fraile, Lorenzo; Chiapponi, Chiara; Amadori, Massimo; Real, Gustavo del; Montoya, María.

In: Journal of virology, Vol. 89, No. 22, 01.01.2015, p. 11190-11202.

Research output: Contribution to journalArticle

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AU - Solórzano, Alicia

AU - Foni, Emanuela

AU - Córdoba, Lorena

AU - Baratelli, Massimiliano

AU - Razzuoli, Elisabetta

AU - Bilato, Dania

AU - del Burgo, María Ángeles Martín

AU - Perlin, David S.

AU - Martínez, Jorge

AU - Martínez-Orellana, Pamela

AU - Fraile, Lorenzo

AU - Chiapponi, Chiara

AU - Amadori, Massimo

AU - Real, Gustavo del

AU - Montoya, María

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Solórzano A, Foni E, Córdoba L, Baratelli M, Razzuoli E, Bilato D et al. Cross-species infectivity of H3N8 influenza virus in an experimental infection in swine. Journal of virology. 2015 Jan 1;89(22):11190-11202. https://doi.org/10.1128/JVI.01509-15