CRP1, a protein localized in filopodia of growth cones, is involved in dendritic growth

Liping Ma, Jeffrey A. Greenwood, Melitta Camartin

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The cysteine-rich protein (CRP) family is a subgroup of LIM domain proteins. CRP1, which cross-links actin filaments to make actin bundles, is the onlyCRPfamily member expressed in the CNS with little known about its function in nerve cells. Here, we report that CRP1 colocalizes with actin in the filopodia of growth cones in cultured rat hippocampal neurons. Knockdown of CRP1 expression by short hairpinRNAinterference results in inhibition of filopodia formation and dendritic growth in neurons. Overexpression of CRP1 increases filopodia formation and neurite branching, which require its actin-bundling activity. Expression ofCRP1with a constitutively active form of Cdc42, a GTPase involved in filopodia formation, increases filopodia formation in COS-7 cells, suggesting cooperation between the two proteins. Moreover, we demonstrate that neuronal activity upregulates CRP1 expression in hippocampal neurons via Ca 2+ influx after depolarization. Ca 2+/calmodulin-dependent protein kinase IV (CaMKIV) and cAMP response element binding protein mediate the Ca 2+-induced upregulation of CRP1 expression. Furthermore, CRP1 is required for the dendritic growth induced by Ca 2+ influx or CaMKIV. Together, these data are the first to demonstrate a role for CRP1 in dendritic growth.

Original languageEnglish (US)
Pages (from-to)16781-16791
Number of pages11
JournalJournal of Neuroscience
Volume31
Issue number46
DOIs
StatePublished - Nov 16 2011

Fingerprint

Growth Cones
Pseudopodia
Calcium-Calmodulin-Dependent Protein Kinase Type 4
Neurons
Actins
Growth
Proteins
Up-Regulation
LIM Domain Proteins
Cyclic AMP Response Element-Binding Protein
Calcium-Calmodulin-Dependent Protein Kinases
COS Cells
GTP Phosphohydrolases
Neurites
Actin Cytoskeleton
Cysteine

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

@article{f16f5c27f54d4ec79534f72429224d2b,
title = "CRP1, a protein localized in filopodia of growth cones, is involved in dendritic growth",
abstract = "The cysteine-rich protein (CRP) family is a subgroup of LIM domain proteins. CRP1, which cross-links actin filaments to make actin bundles, is the onlyCRPfamily member expressed in the CNS with little known about its function in nerve cells. Here, we report that CRP1 colocalizes with actin in the filopodia of growth cones in cultured rat hippocampal neurons. Knockdown of CRP1 expression by short hairpinRNAinterference results in inhibition of filopodia formation and dendritic growth in neurons. Overexpression of CRP1 increases filopodia formation and neurite branching, which require its actin-bundling activity. Expression ofCRP1with a constitutively active form of Cdc42, a GTPase involved in filopodia formation, increases filopodia formation in COS-7 cells, suggesting cooperation between the two proteins. Moreover, we demonstrate that neuronal activity upregulates CRP1 expression in hippocampal neurons via Ca 2+ influx after depolarization. Ca 2+/calmodulin-dependent protein kinase IV (CaMKIV) and cAMP response element binding protein mediate the Ca 2+-induced upregulation of CRP1 expression. Furthermore, CRP1 is required for the dendritic growth induced by Ca 2+ influx or CaMKIV. Together, these data are the first to demonstrate a role for CRP1 in dendritic growth.",
author = "Liping Ma and Greenwood, {Jeffrey A.} and Melitta Camartin",
year = "2011",
month = "11",
day = "16",
doi = "10.1523/JNEUROSCI.2595-11.2011",
language = "English (US)",
volume = "31",
pages = "16781--16791",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "46",

}

CRP1, a protein localized in filopodia of growth cones, is involved in dendritic growth. / Ma, Liping; Greenwood, Jeffrey A.; Camartin, Melitta.

In: Journal of Neuroscience, Vol. 31, No. 46, 16.11.2011, p. 16781-16791.

Research output: Contribution to journalArticle

TY - JOUR

T1 - CRP1, a protein localized in filopodia of growth cones, is involved in dendritic growth

AU - Ma, Liping

AU - Greenwood, Jeffrey A.

AU - Camartin, Melitta

PY - 2011/11/16

Y1 - 2011/11/16

N2 - The cysteine-rich protein (CRP) family is a subgroup of LIM domain proteins. CRP1, which cross-links actin filaments to make actin bundles, is the onlyCRPfamily member expressed in the CNS with little known about its function in nerve cells. Here, we report that CRP1 colocalizes with actin in the filopodia of growth cones in cultured rat hippocampal neurons. Knockdown of CRP1 expression by short hairpinRNAinterference results in inhibition of filopodia formation and dendritic growth in neurons. Overexpression of CRP1 increases filopodia formation and neurite branching, which require its actin-bundling activity. Expression ofCRP1with a constitutively active form of Cdc42, a GTPase involved in filopodia formation, increases filopodia formation in COS-7 cells, suggesting cooperation between the two proteins. Moreover, we demonstrate that neuronal activity upregulates CRP1 expression in hippocampal neurons via Ca 2+ influx after depolarization. Ca 2+/calmodulin-dependent protein kinase IV (CaMKIV) and cAMP response element binding protein mediate the Ca 2+-induced upregulation of CRP1 expression. Furthermore, CRP1 is required for the dendritic growth induced by Ca 2+ influx or CaMKIV. Together, these data are the first to demonstrate a role for CRP1 in dendritic growth.

AB - The cysteine-rich protein (CRP) family is a subgroup of LIM domain proteins. CRP1, which cross-links actin filaments to make actin bundles, is the onlyCRPfamily member expressed in the CNS with little known about its function in nerve cells. Here, we report that CRP1 colocalizes with actin in the filopodia of growth cones in cultured rat hippocampal neurons. Knockdown of CRP1 expression by short hairpinRNAinterference results in inhibition of filopodia formation and dendritic growth in neurons. Overexpression of CRP1 increases filopodia formation and neurite branching, which require its actin-bundling activity. Expression ofCRP1with a constitutively active form of Cdc42, a GTPase involved in filopodia formation, increases filopodia formation in COS-7 cells, suggesting cooperation between the two proteins. Moreover, we demonstrate that neuronal activity upregulates CRP1 expression in hippocampal neurons via Ca 2+ influx after depolarization. Ca 2+/calmodulin-dependent protein kinase IV (CaMKIV) and cAMP response element binding protein mediate the Ca 2+-induced upregulation of CRP1 expression. Furthermore, CRP1 is required for the dendritic growth induced by Ca 2+ influx or CaMKIV. Together, these data are the first to demonstrate a role for CRP1 in dendritic growth.

UR - http://www.scopus.com/inward/record.url?scp=81255195528&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=81255195528&partnerID=8YFLogxK

U2 - 10.1523/JNEUROSCI.2595-11.2011

DO - 10.1523/JNEUROSCI.2595-11.2011

M3 - Article

VL - 31

SP - 16781

EP - 16791

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 46

ER -