Cryo-EM reveals the molecular basis oflaminin polymerization and LN-lamininopathies

Arkadiusz W. Kulczyk, Karen K. McKee, Ximo Zhang, Iwona Bizukojc, Ying Q. Yu, Peter D. Yurchenco

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Laminin polymerization is the major step in basement membranes assembly. Its failures cause laminin N-terminal domain lamininopathies including Pierson syndrome. We have employed cryo-electron microscopy to determine a 3.7 Å structure of the trimeric laminin polymer node containing α1, β1 and γ1 subunits. The structure reveals the molecular basis of calcium-dependent formation of laminin lattice, and provides insights into polymerization defects manifesting in human disease.

Original languageEnglish (US)
Article number317
JournalNature communications
Volume14
Issue number1
DOIs
StatePublished - Dec 2023

All Science Journal Classification (ASJC) codes

  • General Chemistry
  • General Biochemistry, Genetics and Molecular Biology
  • General
  • General Physics and Astronomy

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