Crystal and Molecular Structure of a DNA Fragment Containing a 2-Aminoadenine Modification: The Relationship between Conformation, Packing, and Hydration in Z-DNA Hexamers

Bohdan Schneider, Stephan L. Ginell, Roger Jones, Barbara Gaffney, Helen M. Berman

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

The crystal and molecular structure of d(CGUAʹCG)2(where Aʹis 2-aminoadenine) has been determined and refined to an R factor of 13.8% for data 8.0-1.3 Å. The structure is very similar to the original Z-DNA structures with the sequence d(CGCGCG)2[Gessner, R.V., Frederick, C.A., Quigley, G.J., Rich, A., & Wang, A.H.-J. (1989) J. Biol. Chem. 264, 7921] and shows that the substitution of 2-aminoadenine-uracil base pairs in the two central steps is consistent with Z-DNA formation. In addition, we show how waters mediating intermolecular interactions may help to explain the ZI-ZII conformational pattern found in many Z-DNA structures.

Original languageEnglish (US)
Pages (from-to)9622-9628
Number of pages7
JournalBiochemistry
Volume31
Issue number40
DOIs
StatePublished - Feb 1 1992

All Science Journal Classification (ASJC) codes

  • Biochemistry

Fingerprint

Dive into the research topics of 'Crystal and Molecular Structure of a DNA Fragment Containing a 2-Aminoadenine Modification: The Relationship between Conformation, Packing, and Hydration in Z-DNA Hexamers'. Together they form a unique fingerprint.

Cite this