Crystal and Molecular Structure of a New Z-DNA Crystal Form: D[CGT(2-NH2-A)CG] and Its Platinated Derivative

Gary N. Parkinson, Georgia M. Arvanitis, Leslie Lessinger, Stephan L. Ginell, Roger Jones, Barbara Gaffney, Helen M. Berman

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

The three-dimensional structure of d[CGTA'CG], where A'= [2-NH2-A], was determined to atomic (1.35 Å) resolution by single isomorphous replacement. The d[CGTA'CG] hexamer crystallizes in space group P3221, and is not isomorphous with other DNA hexanucleotides. Despite completely different crystal packing, the essential characterisitics of the Z-DNA conformation are maintained. The structure was determined by single isomorphous replacement using a triammine platinum fragment. Thus, this study also demonstrates, for the first time, the feasibility of the use of this reagent for the direct phasing of DNA crystal structures.

Original languageEnglish (US)
Pages (from-to)15487-15495
Number of pages9
JournalBiochemistry
Volume34
Issue number47
DOIs
StatePublished - Nov 1995

All Science Journal Classification (ASJC) codes

  • Biochemistry

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