Crystal structure of a DNA decamer showing a novel pseudo four-way helix-helix junction

Neil Spink, Christine M. Nunn, Jaroslav Vojtechovsky, Helen M. Berman, Stephen Neidle

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

The crystal structure of the decanucleotide d(CGCAATTGCG)2 has been solved by a combination of molecular replacement and heavy-atom procedures and has been refined to an R factor of 20.2% at 2.7 Å. It is not a fully base-paired duplex but has a central core of eight Watson-Crick base pairs flanked by unpaired terminal guanosines and cytosines. These participate in hydrogen-bonding arrangements with adjacent decamer duplexes in the crystal lattice. The unpaired guanosines are bound in the G+C regions of duplex minor grooves. The cytosines have relatively high mobility, even though they are constrained to be in one region where they are involved in base-paired triplets with G·C base pairs. The 5′-AATT sequence in the duplex region has a narrow minor groove, providing further confirmation of the sequence-dependent nature of groove width.

Original languageEnglish (US)
Pages (from-to)10767-10771
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number23
DOIs
StatePublished - Nov 7 1995

All Science Journal Classification (ASJC) codes

  • General

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