Crystallization and preliminary X-ray studies of recombinant human granulocyte-macrophage colony stimulating factor

Judith M. La Londe, Lewis S. Hanna, Rosemarie Rattoballi, Helen M. Berman, Donald Voet

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Human granulocyte-macrophage colony stimulating factor (hGM-CSF) is an important regulator of growth and differentiation for mononuclear and polymorphonuclear phagocytes. Here we report the crystallization and preliminary X-ray studies of Escherichia coli-expressed hGM-CSF. The crystals are orthorhombic, with the space group P212121, and have unit cell dimensions a = 46.62 A ̊, b = 58.73 A ̊ and c = 126.42 A ̊. Recombinant hGM-CSF crystals diffract X-rays to 2.4 Å resolution and are thus suitable for X-ray structural studies.

Original languageEnglish (US)
Pages (from-to)783-785
Number of pages3
JournalJournal of molecular biology
Volume205
Issue number4
DOIs
StatePublished - Feb 20 1989
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

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